Abstract
Colicins are natural non-antibiotic bacterial proteins with a narrow spectrum but an extremely high antibacterial activity. These proteins are promising food additives for the control of major pathogenic Shiga toxin-producing E. coli serovars in meats and produce. In the USA, colicins produced in edible plants such as spinach and leafy beets have already been accepted by the U. S. Food and Drug Administration (FDA) and U. S. Department of Agriculture (USDA) as food-processing antibacterials through the GRAS (generally recognized as safe) regulatory review process. Nicotiana benthamiana, a wild relative of tobacco, N. tabacum, has become the preferred production host plant for manufacturing recombinant proteins-including biopharmaceuticals, vaccines, and biomaterials-but the purification procedures that have been employed thus far are highly complex and costly. We describe a simple and inexpensive purification method based on specific acidic extraction followed by one chromatography step. The method provides for a high recovery yield of purified colicins, as well as a drastic reduction of nicotine to levels that could enable the final products to be used on food. The described purification method allows production of the colicin...Continue Reading
References
Jul 1, 1987·Journal of Bacteriology·J KöckV Braun
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Nov 26, 2009·Bioresource Technology·H FuY M Lo
Mar 6, 2010·The Plant Journal : for Cell and Molecular Biology·Andrea T ToddJonathan E Page
Dec 21, 2011·Antimicrobial Agents and Chemotherapy·Karen SmithDaniel Walker
Oct 5, 2013·Archives of Toxicology·Bernd Mayer
Oct 16, 2013·Vaccine·James F CummingsUNKNOWN Fraunhofer USA Center for Molecular Biotechnology Study Group
Jul 1, 2014·BioMed Research International·Daniel TuséKaren A McDonald
Aug 30, 2014·Nature·Xiangguo QiuGary P Kobinger
Dec 30, 2014·Frontiers in Microbiology·Shih-Chun YangJia-You Fang
Sep 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Steve SchulzYuri Gleba
Sep 25, 2015·Proceedings of the National Academy of Sciences of the United States of America·Todd R Callaway, Trisha G Sheridan
Oct 2, 2015·BioMed Research International·Daniel TuséJohn E Butler-Ransohoff
Mar 19, 2016·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Stéphane PilletNathalie Landry
Jun 3, 2016·The Biochemical Journal·Laura C McCaugheyDaniel Walker
Oct 30, 2016·MAbs·Somen NandiKaren A McDonald
Jan 1, 2015·Molecular Plant-microbe Interactions : MPMI·Michael M GoodinSteven A Lommel
Oct 4, 2017·PloS One·Šarūnas PaškevičiusAušra Ražanskienė
Citations
Aug 24, 2019·Biotechnology Progress·Matthew J McNultyKaren A McDonald
Jul 10, 2019·Nature Plants·Jennifer A SchmidtBeth A Ahner
Apr 27, 2019·Frontiers in Plant Science·Simone Hahn-LöbmannYuri Gleba
Feb 3, 2021·Pest Management Science·Ray ChaiDaniel Walker
Nov 20, 2020·Plant Physiology and Biochemistry : PPB·Kevin BellandeHervé Canut