Simple radioimmunobinding assay for quantitation of sperm antibodies of IgG immunoglobulin class

American Journal of Reproductive Immunology and Microbiology : AJRIM
G N Clarke


The immunobead test (IBT) is an excellent test for initial sperm antibody screening and evaluation but is impractical when used to quantitate antibody levels using a twofold dilution series. The aim of this study, therefore, was to develop a relatively simple radioimmunobinding assay that would allow quantitation of sperm antibodies of IgG immunoglobulin class, which predominate in male sera. [125I]-Protein G was chosen as the radioligand because it binds to all IgG subclasses, but not to IgA or IgM. The results of the investigation indicate that the [125I]-protein G assay (PGA) allows efficient quantitation of sperm antibodies, as evidenced by a highly significant (P less than .0001) correlation (Spearman's, Rs = 0.94) between the PGA results and IgG-IBT titres.


Jan 1, 1987·American Journal of Reproductive Immunology and Microbiology : AJRIM·G N Clarke
Sep 1, 1986·American Journal of Reproductive Immunology and Microbiology : AJRIM·L A Eng, C B Metz
Mar 1, 1985·American Journal of Reproductive Immunology and Microbiology : AJRIM·G N ClarkeC Smaila
Apr 1, 1985·American Journal of Reproductive Immunology and Microbiology : AJRIM·G N ClarkeW I Johnston
Mar 1, 1980·Fertility and Sterility·L A Hill, J K Hampton
Sep 25, 1980·The New England Journal of Medicine·G G HaasA D Schreiber


Jul 1, 1995·Archives of Andrology·G N ClarkeH W Baker

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