Simple, rapid, and cost-effective microextraction by the packed sorbent method for quantifying of urinary free catecholamines and metanephrines using liquid chromatography-tandem mass spectrometry and its application in clinical analysis

Analytical and Bioanalytical Chemistry
Xin Xiong, Yuanyuan Zhang

Abstract

Pheochromocytoma and paraganglioma (PPGL) are rare neuroendocrine tumors arising from adrenal and extra-adrenal chromaffin cells. They produce excessive amounts of catecholamines and their metabolites. A newly analytical procedure based on the semi-automated microextraction by packed sorbent (MEPS) technique, using a digitally controlled syringe (eVol) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS), was developed to quantify free urinary catecholamines and metanephrines. The important parameters affecting MEPS performance, namely the type of sorbent material (porous graphitized carbon (PGC), polar enhanced polymer (PEP), cation-exchange (CX) and C18), number of extraction cycles, and elution solvent system, were evaluated. The optimal experimental conditions involved the loading of sample mixture in seven extraction cycles through a C18 sorbent in a MEPS syringe, followed by using elution solutions (water/acetonitrile/formic acid, 95/4.75/0.25). The entire sample preparation took about 4 min. Chromatographic separation was well achieved with an HSS PFP column using the gradient elution. The linearity range of the method was 0.167-33.4 ng/mL for epinephrine, 0.650-130 ng/mL for norepinephrine, 1.53-306 n...Continue Reading

References

Feb 1, 2002·The Journal of Clinical Hypertension·William M Manger, Ray W Gifford
Mar 21, 2002·JAMA : the Journal of the American Medical Association·Jacques W M LendersGraeme Eisenhofer
Jun 21, 2003·Clinical Chemistry·Thomas M Annesley
Jun 14, 2005·World Journal of Surgery·Luc A Dubois, Daryl K Gray
Aug 9, 2007·Nature Clinical Practice. Endocrinology & Metabolism·Karel Pacak, Graeme Eisenhofer
Oct 9, 2009·Journal of Chromatography. a·Mohamed Abdel-Rehim
Jun 16, 2010·Journal of Clinical Pathology·Jeffrey Barron
May 18, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Jessica GablerSihe Wang
Nov 1, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zlatuse D Clark, Elizabeth L Frank
Mar 13, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jonathan SimoninEric Grouzmann
Jun 4, 2014·The Journal of Clinical Endocrinology and Metabolism·Jacques W M LendersWilliam F Young
Dec 17, 2014·Journal of Pharmaceutical and Biomedical Analysis·Maria Addolorata SaracinoLaura Mercolini
Nov 12, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Liu YangMohamed Abdel-Rehim
Mar 7, 2017·Clinica Chimica Acta; International Journal of Clinical Chemistry·Zlatuse D ClarkElizabeth L Frank
Sep 25, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhengzhi XieSanjay Srivastava
Dec 21, 2018·Clinica Chimica Acta; International Journal of Clinical Chemistry·Graeme EisenhoferJacques W M Lenders

❮ Previous
Next ❯

Citations

Mar 5, 2021·Biomedical Chromatography : BMC·Seyed Mosayeb DaryanavardMohamed Abdel-Rehim
Aug 29, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Lijun SongZhiliang Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.