PMID: 9187408Apr 25, 1997Paper

Simple, rapid and sensitive method for the determination of indomethacin in plasma by high-performance liquid chromatography with ultraviolet detection

Journal of Chromatography. B, Biomedical Sciences and Applications
J SatoK Ito

Abstract

A micro method for determination of indomethacin in plasma was developed. Following deproteinization of plasma with acetonitrile containing internal standard (mefenamic acid), the separation of indomethacin and internal standard was achieved by high-performance liquid chromatography using a 7 microm LiChrosorb-RP18 column (250x4 mm I.D.) at 50 degrees C. The mobile phase was 6 mM phosphoric acid-acetonitrile (50:50). The flow-rate was kept at 2.0 ml/min and the column effluent was monitored at 205 nm. The coefficients of variation of the method estimated at 0.2 and 1.0 microg/ml were 4.2 and 2.3%, and the detection limit of the drug was about 0.05 microg/ml (S/N=5). The method requires minimum pretreatment of the plasma with a small sample volume (25 microl), and is very suitable for therapeutic drug monitoring of indomethacin in premature infants with symptomatic patent ductus arteriosus.

References

Oct 27, 1989·Journal of Chromatography·A Avgerinos, S Malamataris
Aug 12, 1983·Journal of Chromatography·A C Mehta, R T Calvert

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Citations

Feb 28, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shicheng LiuSatoshi Takayama
Oct 31, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·S HessK Eger
Sep 14, 2001·Journal of Veterinary Pharmacology and Therapeutics·J C BoggioQ McKellar
May 29, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·M C HamoudiA Bochot
Feb 26, 2008·British Journal of Pharmacology·D SodiniF Coceani
Jun 29, 2016·European Journal of Drug Metabolism and Pharmacokinetics·Mikhail V KhvostovSergey V An'kov

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