Simple rapid in vitro screening method for SARS-CoV-2 anti-virals that identifies potential cytomorbidity-associated false positives.

Virology Journal
Kexin YanAndreas Suhrbier

Abstract

The international SARS-CoV-2 pandemic has resulted in an urgent need to identify new anti-viral drugs for treatment of COVID-19. The initial step to identifying potential candidates usually involves in vitro screening that includes standard cytotoxicity controls. Under-appreciated is that viable, but stressed or otherwise compromised cells, can also have a reduced capacity to replicate virus. A refinement proposed herein for in vitro drug screening thus includes a simple growth assay to identify drug concentrations that cause cellular stress or "cytomorbidity", as distinct from cytotoxicity or loss of viability. A simple rapid bioassay is presented for antiviral drug screening using Vero E6 cells and inhibition of SARS-CoV-2 induced cytopathic effects (CPE) measured using crystal violet staining. We use high cell density for cytotoxicity assays, and low cell density for cytomorbidity assays. The assay clearly illustrated the anti-viral activity of remdesivir, a drug known to inhibit SARS-CoV-2 replication. In contrast, nitazoxanide, oleuropein, cyclosporine A and ribavirin all showed no ability to inhibit SARS-CoV-2 CPE. Hydroxychloroquine, cyclohexamide, didemnin B, γ-mangostin and linoleic acid were all able to inhibit viral ...Continue Reading

References

Jul 1, 1967·The American Journal of the Medical Sciences·S J Updike, P L Eichman
Dec 31, 2002·Toxicology·J A Pietenpol, Z A Stewart
Aug 14, 2010·PLoS Neglected Tropical Diseases·Vianney TricouCameron P Simmons
May 10, 2011·The Lancet Infectious Diseases·Nicholas I PatonAnnelies Wilder Smith
Jul 6, 2019·Science·Philippe Pierre
Mar 10, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Xueting YaoDongyang Liu
Apr 7, 2020·Antiviral Research·Leon CalyKylie M Wagstaff
May 24, 2020·The New England Journal of Medicine·John H BeigelUNKNOWN ACTT-1 Study Group Members
May 30, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C RodrigoS Rajapakse
Jun 3, 2020·Journal of Biochemistry·Ryan HoustonYusuke Sekine
Jun 10, 2020·Nature·Brandi N WilliamsonEmmie de Wit
Jul 21, 2020·International Journal of Antimicrobial Agents·Mohammad Sultan Khuroo
Jul 23, 2020·Nature·Markus HoffmannStefan Pöhlmann
Aug 28, 2020·Medicinal Research Reviews·Thanigaimalai PillaiyarMaheswaran Easwaran
Sep 5, 2020·Journal of General Internal Medicine·Arunmozhimaran ElavarasiPramod Garg
Sep 7, 2020·The FEBS Journal·Stephen E GirardinDamien Arnoult
Sep 10, 2020·Viruses·Nina EiermannAlessia Ruggieri
Sep 15, 2020·Frontiers in Endocrinology·Hui-Chen Ku, Ching-Feng Cheng
Sep 18, 2020·Nucleic Acids Research·Anna Constance VindSimon Bekker-Jensen
Sep 23, 2020·Science·Christine ToelzerChristiane Schaffitzel
Jun 10, 2021·Nature Communications·Alberto A AmarillaAlexander A Khromykh

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Citations

Aug 28, 2021·International Journal of Molecular Sciences·Maamoun BasheerNimer Assy

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