Simple spectrophotometric method for determination of paroxetine in tablets using 1,2-naphthoquinone-4-sulphonate as a chromogenic reagent

International Journal of Analytical Chemistry
Ibrahim DarwishLama I Al-Rayes


Simple and rapid spectrophotometric method has been developed and validated for the determination of paroxetine (PRX) in tablets. The proposed method was based on nucleophilic substitution reaction of PRX with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium to form an orange-colored product of maximum absorption peak (lambda(max)) at 488 nm. The stoichiometry and kinetics of the reaction were studied, and the reaction mechanism was postulated. Under the optimized reaction conditions, Beer's law correlating the absorbance (A) with PRX concentration (C) was obeyed in the range of 1-8 mug mL(-1). The regression equation for the calibration data was: A = 0.0031 + 0.1609 C, with good correlation coefficients (0.9992). The molar absorptivity (epsilon) was 5.9 x 10(5) L mol(-1) 1 cm(-1). The limits of detection and quantitation were 0.3 and 0.8 mug mL(-1), respectively. The precision of the method was satisfactory; the values of relative standard deviations did not exceed 2%. The proposed method was successfully applied to the determination of PRX in its pharmaceutical tablets with good accuracy and precisions; the label claim percentage was 97.17 +/- 1.06 %. The results obtained by the proposed method were comparable with...Continue Reading


Jan 6, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·C T LaiG B Baker
May 17, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·L LabatJ P Dubost
Oct 4, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hans Jörg LeisGünter Fauler
Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhimeng Zhu, Len Neirinck
Jun 12, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Isis A ZainaghiRegina H C Queiroz
Oct 13, 2004·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Lixiao XuYan Xiao
Jan 6, 2005·Journal of Personality and Social Psychology·Jamin Halberstadt
Jun 13, 2006·Journal of Pharmaceutical and Biomedical Analysis·Henri P A NouwsJosé A Rodrigues
Nov 18, 2006·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Quanmin Li, Zhanjun Yang

Related Concepts

6-Aminocaproic Acid
Molar Tooth
Spectrophotometry, Ultraviolet
Carmoisine A
Orange (Fruit)
Stents, Vascular, Coronary
Determination Aspects
Pharmacologic Substance

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.