Simple Synthetic Molecular Hydrogels from Self-Assembling Alkylgalactonamides as Scaffold for 3D Neuronal Cell Growth

ACS Applied Materials & Interfaces
Anaïs ChalardJuliette Fitremann

Abstract

In this work, we demonstrated that the hydrogel obtained from a very simple and single synthetic molecule, N-heptyl-galactonamide was a suitable scaffold for the growth of neuronal cells in 3D. We evidenced by confocal microscopy the presence of the cells into the gel up to a depth of around 200 μm, demonstrating that the latter was permissive to cell growth and enabled a true 3D colonization and organization. It also supported successfully the differentiation of adult human neuronal stem cells (hNSCs) into both glial and neuronal cells and the development of a really dense neurofilament network. So the gel appears to be a good candidate for neural tissue regeneration. In contrast with other molecular gels described for cell culture, the molecule can be obtained at the gram scale by a one-step reaction. The resulting gel is very soft, a quality in accordance with the aim of growing neuronal cells, that requires low modulus substrates similar to the brain. But because of its fragility, specific procedures had to be implemented for its preparation and for cell labeling and confocal microscopy observations. Notably, the implementation of a controlled slow cooling of the gel solution was needed to get a very soft but nevertheless c...Continue Reading

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Citations

Oct 17, 2019·Soft Matter·Kenji AramakiCosima Stubenrauch
Jul 28, 2019·Brain Research Bulletin·Angelo AccardoIsabelle Loubinoux
Jul 2, 2021·Journal of Colloid and Interface Science·Delphine BordignonJuliette Fitremann
Aug 3, 2021·Chemical Reviews·Lu SuGuosong Chen
Aug 12, 2021·Chemical Communications : Chem Comm·Daniel McDowallDave J Adams
Dec 23, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Omar El HamouiBruno Alies
Jul 22, 2020·Current Stem Cell Research & Therapy·Ali GolchinSimzar Hosseinzadeh

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