Simple two-step procedure for the preparation of highly active pure equine milk lysozyme

Journal of Chromatography. a
W NoppeM De Cuyper

Abstract

A fast, simple two-step purification scheme is presented for the isolation of lysozyme at a high yield from equine milk. In the first step, fluidized bed technology, using the Streamline system, was exploited. In the following step, advantage was taken of Ca(2+)-induced conformational changes to obtain a pure, high specific activity, enzyme fraction by hydrophobic interaction chromatography.

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Citations

Mar 11, 2000·Biotechnology and Bioengineering·R Ghosh, Z F Cui
Mar 17, 1999·Journal of Molecular Recognition : JMR·H A Chase
Aug 5, 2009·Protein Engineering, Design & Selection : PEDS·Vida CasaiteRolandas Meskys
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Aug 2, 2003·Biochemical and Biophysical Research Communications·Rodolfo ValdésCarlos Borroto
Nov 19, 2014·Chembiochem : a European Journal of Chemical Biology·Jørn D KaspersenJan Skov Pedersen
May 25, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fernanda G De FeliceSérgio T Ferreira
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Jun 6, 1998·Biochemistry·P HaezebrouckH Van Dael
Dec 10, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·M Kaufmann

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