Simple wound exudate collection method identifies bioactive cytokines and chemokines in (arterio) venous ulcers

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Kim L KroezeSusan Gibbs

Abstract

A major challenge for clinicians treating (arterio) venous leg ulcers is to decide between standard therapy and advanced interventions. Here, we developed a simple method to collect human material representative of the ulcer wound bed, which can be used to identify biomarkers for prognostic test development. Superficial surgical debridement was performed using a small vidal curette during the weekly visit to the outpatient clinic. Moist, easily removable debridement material essentially blood free (including necrotic and nonviable slough) was collected from the surface of the ulcer. The amount ranged from 5.5 mg to 78 mg material per ulcer. Seventeen cytokines, chemokines, and growth factors were extracted and analyzed by enzyme-linked immunosorbent assay (concentration range: 0.0005-78 ng/mg total protein). Notably, CXCL8 was by far the most abundant protein present. Inflammatory mediators were more abundant than anti-inflammatory mediators (e.g., interleukin (IL)-10 and transforming growth factor-β1). Bioactivity assays showed chronic wound extracts to be capable of stimulating fibroblast migration in a chemokine-dependent manner and also capable of stimulating healthy cells within skin substitutes to secrete wound healing me...Continue Reading

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Citations

Feb 24, 2016·Journal of Biomechanics·Hanneke N MonsuurSusan Gibbs
Dec 3, 2015·Colloids and Surfaces. B, Biointerfaces·Shixuan ChenLin Zhang
Feb 27, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Takeo MinematsuHiromi Sanada
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Oct 19, 2016·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Christa J BodaanChristine Theoret
Jul 25, 2015·Vascular Medicine·Nasim HedayatiDaniel Link
Apr 8, 2017·Journal of Tissue Engineering and Regenerative Medicine·Mireille A BoinkSusan Gibbs
Apr 11, 2019·Journal of Tissue Engineering and Regenerative Medicine·Charlotte Rodrigues NevesSusan Gibbs
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Feb 9, 2021·Advances in Wound Care·Xinling BiQing-Sheng Mi
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Dec 31, 2020·Journal of Clinical Medicine·Joseph D RaffettoFerdinando Mannello

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