Simplified and improved somatic embryogenesis for clonal propagation of Pinus pinaster (Ait.)

Plant Cell Reports
Marie-Anne Lelu-WalterKrystyna Klimaszewska

Abstract

In this study, several improvements and simplifications of SE protocols in Pinus pinaster (Ait.), a species of economic importance in the regions of Western Europe, are described. These improvements pertained to all stages of SE including high initiation frequencies in eight control pollinated seed families, relatively high somatic embryo maturation yield when cells were coated with particles of activated charcoal and a rapid production of plants directly in a shade house. The SE initiation frequency from isolated zygotic embryos was high (up to 100%) and plants were produced from 11 embryogenic lines representing all crosses. Based on these results, the estimated number of somatic embryos required to produce 1,000 plants varied from slightly more than the required number of plants to more than double this number depending on the line. Such an estimate is critical in developing plant production strategy when a number of embryogenic lines are considered for production of clonal plants.

References

Mar 15, 2000·British Dental Journal·H R Chapman, N C Kirby-Turner
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·A RamarosandratanaM Pâques
Apr 19, 2002·Tree Physiology·Patrick von AderkasMarie-Anne Lelu
Sep 2, 2004·Tree Physiology·Anna-Maija NiskanenAri Pappinen
May 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y S ParkJ M Bonga

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Citations

Jan 1, 2014·TheScientificWorldJournal·José M Alvarez, Ricardo J Ordás
Aug 12, 2009·Journal of Biotechnology·Liliana MarumCélia Miguel
Sep 13, 2008·Biotechnology Advances·T Dennis Thomas
Nov 22, 2013·Plant Biotechnology Journal·Javier CanalesFrancisco M Cánovas
Nov 13, 2009·Plant Biotechnology Journal·Andrew R RobinsonShawn D Mansfield
Mar 7, 2019·Frontiers in Plant Science·Isabel ArrillagaEster Sales
Nov 22, 2018·In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association·Nazmul H A MamunUlrika Egertsdotter
Feb 13, 2019·Frontiers in Plant Science·Tsuyoshi E Maruyama, Yoshihisa Hosoi

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