Simplified and versatile method for isolation of high-quality RNA from pancreas.

BioTechniques
Michelle GriffinAliye Uc

Abstract

Isolation of high-quality RNA from pancreas is challenging because the organ contains large quantities of RNases and undergoes autolysis upon harvest. Here we present a simplified perfusion method of the pancreas using an RNase inhibitor. The technique consistently yields high-quality RNA from stored pancreas samples suitable for molecular biology applications, including quantitative RT-PCR. Yields are comparable to RNA isolated from pancreas immediately, but superior to RNA isolated from stored samples that were snap-frozen or immersed in an RNase inhibitor solution. In addition, when compared to the previously reported in situ ductal perfusion technique, our method does not cause histological artifacts.

References

Dec 1, 1996·Molecular Biotechnology·S S GillT I Matula
Feb 14, 2006·Molecular Aspects of Medicine·Simone Fleige, Michael W Pfaffl
Jun 19, 2007·Pancreas·Takayoshi KibaYasuhito Ishigaki
Oct 22, 2008·Applied Biochemistry and Biotechnology·Dongmin LiShemin Lu

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Citations

May 21, 2013·Nature Methods·Xian AdiconisJoshua Z Levin
Nov 27, 2019·Journal of Virological Methods·Paola ModestoSimone Peletto
Apr 11, 2019·Analytica Chimica Acta·Kelin WangKermit K Murray
Feb 21, 2016·Analytical Biochemistry·Cécile AugereauPatrick Jacquemin
Jun 1, 2021·Physiological Genomics·Patrick B SchwartzSean M Ronnekleiv-Kelly

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