Simplified Bioprinting-Based 3D Cell Culture Infection Models for Virus Detection.

Viruses
Robert KobanMarkus Neumann

Abstract

Studies of virus-host interactions in vitro may be hindered by biological characteristics of conventional monolayer cell cultures that differ from in vivo infection. Three-dimensional (3D) cell cultures show more in vivo-like characteristics and may represent a promising alternative for characterisation of infections. In this study, we established easy-to-handle cell culture platforms based on bioprinted 3D matrices for virus detection and characterisation. Different cell types were cultivated on these matrices and characterised for tissue-like growth characteristics regarding cell morphology and polarisation. Cells developed an in vivo-like morphology and long-term cultivation was possible on the matrices. Cell cultures were infected with viruses which differed in host range, tissue tropism, cytopathogenicity, and genomic organisation and virus morphology. Infections were characterised on molecular and imaging level. The transparent matrix substance allowed easy optical monitoring of cells and infection even via live-cell microscopy. In conclusion, we established an enhanced, standardised, easy-to-handle bioprinted 3D-cell culture system. The infection models are suitable for sensitive monitoring and characterisation of virus-...Continue Reading

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Citations

Jun 26, 2021·Journal of Biomedical Nanotechnology·Wei JiangShuyan Zhao

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Methods Mentioned

BETA
chips
bioprinting
bioprinter
biopsy
Assay
PCR
fluorescence microscopy

Software Mentioned

Zen
control
Rhinoceros
Fiji

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