Simplified enzyme-linked immunosorbent assay for specific antibodies to respiratory syncytial virus.

Journal of Clinical Microbiology
L VaurF Bricout

Abstract

A simplified and reliable enzyme-linked immunosorbent assay (ELISA) was applied to the detection of serum antibodies against respiratory syncytial virus (RSV). RSV-infected cells were fixed and dried on 96-well microtiter plates and kept at 4 degrees C. The titers of reference sera were determined by endpoint dilution. A linear relation was found between the titers and the logarithm of absorbance values of sera diluted to 1:1,000 (r = 0.93, P less than 0.001). Measurement of RSV antibodies was done by using a single serum dilution (1:1,000) in conjunction with a standard curve. A strong correlation was found between complement fixation and ELISA results (r = 0.89, P less than 0.001). In addition, the ELISA method exhibited higher titers and a greater sensitivity than did complement fixation, although the applicability of the assay is limited with positive serum samples of low titer.

References

Mar 1, 1979·Proceedings of the Society for Experimental Biology and Medicine·P O LeinikkiJ L Sever
Jun 1, 1983·The Journal of Infectious Diseases·G P RiceM B Oldstone
Sep 1, 1980·Journal of Clinical Microbiology·M C SteinhoffK C Schnabel
Apr 1, 1961·Proceedings of the Society for Experimental Biology and Medicine·V V HAMPARIANJ STOKES

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