Simplified Intestinal Microbiota to Study Microbe-Diet-Host Interactions in a Mouse Model.

Cell Reports
Petia Kovatcheva-DatcharyF Bäckhed

Abstract

The gut microbiota can modulate human metabolism through interactions with macronutrients. However, microbiota-diet-host interactions are difficult to study because bacteria interact in complex food webs in concert with the host, and many of the bacteria are not yet characterized. To reduce the complexity, we colonize mice with a simplified intestinal microbiota (SIM) composed of ten sequenced strains isolated from the human gut with complementing pathways to metabolize dietary fibers. We feed the SIM mice one of three diets (chow [fiber rich], high-fat/high-sucrose, or zero-fat/high-sucrose diets [both low in fiber]) and investigate (1) how dietary fiber, saturated fat, and sucrose affect the abundance and transcriptome of the SIM community, (2) the effect of microbe-diet interactions on circulating metabolites, and (3) how microbiota-diet interactions affect host metabolism. Our SIM model can be used in future studies to help clarify how microbiota-diet interactions contribute to metabolic diseases.

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Citations

Aug 6, 2019·Cell Host & Microbe·Dominic A ColosimoSean F Brady
Jan 19, 2021·International Journal of Medical Microbiology : IJMM·Aida IljazovicTill Strowig
Mar 5, 2021·Cell Host & Microbe·Bahtiyar YilmazAndrew J Macpherson
Mar 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jun GengJens Nielsen
Apr 1, 2021·Microbes and Infection·Giuliano BayerDana J Philpott
Jun 26, 2021·Frontiers in Microbiology·Vincent G Martinson, Michael R Strand
Aug 29, 2021·Nature Reviews. Gastroenterology & Hepatology·Daniel A SchupackPurna C Kashyap
Oct 16, 2021·Current Opinion in Pediatrics·Maryam Ali Al-NesfVidya Mohamed-Ali

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Datasets Mentioned

BETA
PRJEB22735

Methods Mentioned

BETA
PCR
RNA-seq
Illumina sequencing
Illumina

Software Mentioned

EchoMRI
count
HMMER
HTSeq
GraphPad Prism
STAR
Samtools
Circos
Hotpep
DIAMOND

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