PMID: 2096914May 1, 1990Paper

Simplified method for conjugating macrocyclic bifunctional chelating agents to antibodies via 2-iminothiolane

Bioconjugate Chemistry
M J McCallC F Meares

Abstract

A one-step method for conjugating macrocyclic chelators to antibodies using the protein modification reagent 2-iminothiolane controls aggregation, maintains immunoreactivity, and produces consistent chelate/antibody ratios. Conjugation conditions have been investigated with the macrocyclic chelates 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane-N,N ',N",N"'-tetraacetic acid and 2-[p-(bromoacetamido)benzyl]-1,4,7,10-tetraazacyclododecane-N,N',N ",N"'-tetraacetic acid, with three different monoclonal antibodies. The bifunctional chelating agents are prepared by bromoacetylation of their amine precursors using a two-phase H2O/CHCl3 system, which improves product purity.

Citations

Jun 24, 1998·Annals of Nuclear Medicine·M N HosonoH J Biersack
Nov 1, 1996·Nuclear Medicine and Biology·P J BlowerJ Zweit
Jun 11, 1999·Nuclear Medicine and Biology·C WuM W Brechbiel
Jan 11, 2000·Nuclear Medicine and Biology·G R MirickG L DeNardo
Nov 11, 2008·Bioconjugate Chemistry·Jai Woong SeoKatherine W Ferrara
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·J M ConnettM J Welch
Apr 8, 2004·Cancer Biotherapy & Radiopharmaceuticals·Gerald L DeNardo, Sally J DeNardo
May 6, 2008·Cancer Biotherapy & Radiopharmaceuticals·Joerg LehmannSally J Denardo
Jan 18, 2006·Acta Pharmacologica Sinica·Jun LiZhao-lai Chen
Jul 1, 1996·Photochemistry and Photobiology·R S PanduranglW A Volkert
Jun 13, 2009·Journal of Receptor and Signal Transduction Research·Alex N Eberle, Gabriele Mild
Aug 6, 2008·Nuclear Medicine and Biology·Marie-Laure BiechlinAmaury du Moulinet d'Hardemare
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·H P KalofonosA A Epenetos
Sep 13, 2006·International Journal of Radiation Oncology, Biology, Physics·Joerg LehmannSally J DeNardo
Feb 27, 2015·Bioconjugate Chemistry·Jason L J DearlingAlan B Packard
Aug 23, 2011·Biomacromolecules·Ryan G Wylie, Molly S Shoichet
Mar 1, 1992·Bioconjugate Chemistry·T J McMurryO A Gansow
Jan 1, 1991·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·G DeNardoC Meares
Nov 19, 2019·Chemical Communications : Chem Comm·Charmaine HeeK Swaminathan Iyer
Aug 28, 2019·Science Advances·Koichi SasakiJeffrey A Hubbell
Oct 6, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sally J DeNardoRobert Ivkov
Oct 6, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sally J DeNardoGerald L DeNardo
Jun 19, 2021·Nature Nanotechnology·Niels DammesDan Peer
Sep 13, 2017·Chemical Reviews·Madduri Srinivasarao, Philip S Low

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.