PMID: 279904Sep 1, 1978

Simplified method for purification of ribonuclease H from calf thymus

Proceedings of the National Academy of Sciences of the United States of America
J G Stavrianopoulos, E Chargaff

Abstract

An improved purification procedure for the isolation of ribonuclease H(hybrid nuclease; RNA-DNA-hybrid ribonucleotidohydrolase, EC 3.1.4.34) from the thymus of 4-to 6-months-old calves yields two highly active forms of the enzyme, designated as ribonuclease H1 and H2. Their isoelectric points are 5.0 +/- 0.05 and 5.25 +/- 0.05, respectively; their molecular weight, estimated from gel filtration, is in both cases 64,000 +/- 2000. On sodium dodecyl sulfate gel electrophoresis, two principal bands were identified, with molecular weights of 32,000 and 21,000. The nature of the nucleotides at the 3'-OH terminals, produced initially by the enzymic hydrolysis of hybridized RNA, was examined and shown to be a function of the divalent metal ion employed as activator.

References

Oct 27, 1981·Biochimica Et Biophysica Acta·J G Stavrianopoulos, E Chargaff
Sep 11, 1979·Nucleic Acids Research·H Donis-Keller
Oct 1, 1983·Bioscience Reports·J N Hawthorne
Jul 29, 2010·Journal of the History of Biology·Miguel García-Sancho
Nov 5, 2016·History and Philosophy of the Life Sciences·Michael Fry

Citations

May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J G Starvianopoulos, E Chargaff
Mar 3, 1975·European Journal of Biochemistry·W Büsen, P Hausen
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J G StavrianopoulosE Chargaff
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J G StavrianopoulosE Chargaff
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J G Stavrianopoulos, E Chargaff
Mar 16, 1964·Biochimica Et Biophysica Acta·A POLSONF J JOUBERT

Related Concepts

Structure of Calf of Leg
Cations, Divalent
Ribonuclease H1
Neoplasm of Uncertain or Unknown Behavior of Thymus
SDS-PAGE
Cattle calf (organism)
Bos taurus
Disease of Thymus Gland
RNASEH1
Thymic Tissue

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