PMID: 9174267Apr 11, 1997Paper

Simplified method for the determination of 25-hydroxy and 1alpha,25-dihydroxy metabolites of vitamins D2 and D3 in human plasma. Application to nutritional studies

Journal of Chromatography. B, Biomedical Sciences and Applications
A TakeuchiT Kobayashi

Abstract

A simplified method for the determination of 25-hydroxy and 1alpha,25-dihydroxy metabolites of vitamins D2 and D3 in human plasma was developed. Plasma samples were deproteinizated and applied to a Bond Elut C18OH cartridge to separate 25-hydroxyvitamin D (25-OH-D) and 1alpha,25-dihydroxyvitamin D [1,25(OH)2D] fractions. The 25-OH-D fraction was purified by a Bond Elut C18 cartridge and 25-OH-D2 and 25-OH-D3 were assayed by HPLC using a Zorbax SIL column. The 1,25(OH)2D fraction obtained above was subsequently applied to HPLC using a Zorbax SIL column to separate 1,25(OH)2D2 and 1,25(OH)2D3 fractions which were determined by a radioreceptor assay (RRA) using calf thymus receptor. The method was applied to nutritional studies.

References

Jan 1, 1979·Vitamins and Hormones·D E Lawson, M Davie
Dec 3, 1992·The New England Journal of Medicine·M C ChapuyP J Meunier
Feb 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R EastellB L Riggs
Dec 28, 1989·The New England Journal of Medicine·E A KrallB Dawson-Hughes
Jan 1, 1984·The Journal of Clinical Endocrinology and Metabolism·T A ReinhardtB W Hollis
Jan 1, 1981·Journal of Nutritional Science and Vitaminology·E KurodaT Matsuo
Mar 1, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y WatanabeY Seino
Apr 1, 1993·Journal of Nutritional Science and Vitaminology·S MasudaT Kobayashi

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Citations

Sep 9, 2011·Bioanalysis·Mihaela L Musteata, Florin Marcel Musteata
Oct 23, 2008·Scandinavian Journal of Clinical and Laboratory Investigation·Jette JakobsenLars Ovesen
Jan 5, 2002·Journal of Chromatography. a·K ShimadaT Higashi

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