Simplified reversed-phase HPLC method with spectrophotometric detection for the assay of verapamil in rat plasma

Journal of Pharmaceutical and Biomedical Analysis
C A Lau-Cam, D Piemontese

Abstract

A high-performance liquid chromatographic (HPLC) method was developed for the assay of verapamil in rat plasma. After deproteinization of the plasma sample with an acetonitrile-perchloric acid (8:2) mixture containing dextromethorphan, the internal standard, an aliquot of the supernatant was directly analyzed on a cyanopropylsilane column with methanol-acetonitrile-triethylamine acetate buffer (10:30:60) as the mobile phase and detection at 235 mm. At a flow rate of 1.5 ml min-1, a complete analysis was completed in less than 6 min. The method was linear for verapamil concentrations in the range 0.5-10 micrograms ml-1 (r = 0.9999). Recoveries for the same drug concentrations from spiked rat plasma ranged from 85.6-93.0% (n = 8). The mean RSD values for intraday and interday assay reproducibility (n = 3) were, in both cases, less than 0.9%. The limit of detectability was about 0.1 microgram ml-1. The method was found useful to monitor the plasma levels of verapamil in rats that had received this drug by the nasal, oral and intravenous routes of administration.

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Citations

Aug 23, 2002·Journal of Pharmaceutical and Biomedical Analysis·M WallesJ Pawliszyn
Nov 1, 2002·Journal of Pharmaceutical and Biomedical Analysis·Ensaf Aboul KasimMahasen M Ahmed
Nov 2, 2007·Clinical and Experimental Pharmacology & Physiology·Man ChenHui Wang
Jul 18, 2006·Drug Metabolism and Drug Interactions·Thomas Broeng EjsingKristian Linnet
Nov 6, 2007·Journal of Biochemical and Biophysical Methods·Violeta IvanovaTrajce Stafilov
Sep 7, 2007·Journal of Pharmaceutical Sciences·Hao ZouShen Gao

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