Simulated microgravity affects growth of Escherichia coli and recombinant beta-D-glucuronidase production

Applied Biochemistry and Biotechnology
Liang XiangYuanDa Jiang

Abstract

Effects of simulated microgravity (SMG) on bacteria have been studied in various aspects. However, few reports are available about production of recombinant protein expressed by bacteria in SMG. In this study growth of E. coli BL21 (DE3) cells transformed with pET-28a (+)-pgus in double-axis clinostat that could model low shear SMG environment and the recombinant beta-D-glucuronidase (PGUS) expression have been investigated. Results showed that the cell dry weights in SMG were 16.47%, 38.06%, and 28.79% more than normal gravity (NG) control, and the efficiency of the recombinant PGUS expression in SMG were 18.33%, 19.36%, and 33.42% higher than that in NG at 19 degrees C, 28 degrees C, and 37 degrees C, respectively (P < 0.05).

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Citations

Mar 7, 2014·Tissue Engineering. Part B, Reviews·Daniela GrimmJohann Bauer
Aug 12, 2014·BioMed Research International·Claudia UlbrichDaniela Grimm
Apr 17, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pingping LiQing Ge
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Jul 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Lichun GuoWei Zhao
May 4, 2021·Frontiers in Microbiology·Xinyi ChenHu Liu

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