Simulated microgravity alters differentiation and increases apoptosis in human follicular thyroid carcinoma cells

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Daniela GrimmAugusto Cogoli

Abstract

This study focuses on the effects of simulated microgravity (0g) on the human follicular thyroid carcinoma cell line ML-1. Cultured on a three-dimensional clinostat, ML-1 cells formed three-dimensional MCTSs (MCTS diameter: 0.3 +/- 0.01 mm). After 24 and 48 h of clinorotation, the cells significantly decreased fT3 and fT4 secretion but up-regulated the thyroid-stimulating hormone-receptor expression as well as the production of vimentin, vinculin, and extracellular matrix proteins (collagen I and III, laminin, fibronectin, chondroitin sulfate) compared with controls. Furthermore, ML-1 cells grown on the clinostat showed elevated amounts of the apoptosis-associated Fas protein, of p53, and of bax but showed reduced quantities of bcl-2. In addition, signs of apoptosis became detectable, as assessed by terminal deoxynucleotidyl transferase-mediated dUTP digoxigenin nick end labeling, 4', 6-diamidino-2-phenylindole staining, DNA laddering, and 85-kDa apoptosis-related cleavage fragments. These fragments resulted from enhanced 116-kDa poly(ADP-ribose)polymerase (PARP) activity and apoptosis. These observations suggest that clinorotation elevates intermediate filaments, cell adhesion molecules, and extracellular matrix proteins and s...Continue Reading

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Citations

Feb 3, 2011·European Journal of Applied Physiology·Chun-Yan KangChang-Ting Liu
Jan 11, 2013·International Journal of Molecular Sciences·Jessica PietschDaniela Grimm
Jun 3, 2014·Cell Communication and Signaling : CCS·Elisabeth WarnkeDaniela Grimm
Mar 9, 2006·Radiation and Environmental Biophysics·Lorenzo Manti
Oct 9, 2015·FEBS Open Bio·Danilo RanieriMaria Rosaria Torrisi
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Feb 7, 2008·Journal of Cellular Biochemistry·Claudia UlbrichDaniela Grimm
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Sep 24, 2005·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·Junko KitamotoMakoto Asashima
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Oct 30, 2008·Cell Communication and Signaling : CCS·Oliver UllrichKerstin Lang
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Oct 30, 2016·Basic & Clinical Pharmacology & Toxicology·Ganna AleshchevaDaniela Grimm
May 5, 2017·International Journal of Molecular Sciences·Elisabetta AlbiFrancesco Curcio
Mar 30, 2018·Stem Cells and Development·Daniela GrimmAlamelu Sundaresan
Feb 20, 2009·Tissue Engineering. Part a·Daniela GrimmJohann Bauer
Mar 7, 2014·Tissue Engineering. Part B, Reviews·Daniela GrimmJohann Bauer

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