Simulated microgravity attenuates myogenic differentiation via epigenetic regulations

NPJ Microgravity
Takuma FurukawaLouis Yuge

Abstract

The molecular mechanisms involved in myogenic differentiation are relatively well-known. Myogenic differentiation is regulated by the sequential activation of the basic helix-loop-helix myogenic regulatory transcription factors (MRFs), and biomechanical signals play an important role in the regulation of myogenesis. In this study, we sought to determine whether simulated microgravity culture using Gravite® may affect myoblast differentiation and expression of MRF genes. Although rat myoblasts, L6 cells were differentiated to myotubes in an incubation period-dependent manner, myogenesis of L6 cells was significantly attenuated under simulated microgravity (10-3G) conditions. Real-time Reverse transcription polymerase chain reaction (RT-PCR) showed that expressions of Myog, Myf6, Mef2c, Des, and Ckm under 1 G conditions increase in an incubation period-dependent manner, and that Myod1 expression was specifically observed to increase transiently in the early phase. However, expressions of Myod1 and Myog were significantly inhibited under simulated microgravity conditions. To clarify the molecular mechanisms, L6 cells were treated with 5-AzaC, and further incubated with differentiation medium under 1 G or 10-3 G conditions. The res...Continue Reading

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Citations

Jul 7, 2018·Stem Cells and Development·Takeshi ImuraLouis Yuge
Jan 31, 2019·Stem Cells and Development·Eleonora N Grigoryan, Elena A Radugina
Sep 25, 2019·Journal of Cellular Physiology·Daniela CalziaPaolo Degan
Dec 2, 2020·International Journal of Molecular Sciences·Alessandra CazzanigaJeanette A Maier
Dec 29, 2020·Biochemistry and Biophysics Reports·Hiroaki MatsushitaYukio Ando
Jan 28, 2022·Scientific Reports·Ryo YuzawaYumiko Oishi

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Datasets Mentioned

BETA
ZL02830112.9

Methods Mentioned

BETA
PCR
Transfection
Assay

Software Mentioned

UCSC Genome Browser
GRAVITY CONTROLLER
StatView
ImageJ
Excel

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