Simulated microgravity increases heavy ion radiation-induced apoptosis in human B lymphoblasts

Life Sciences
Bingrong DangChangjun Lin

Abstract

Microgravity and radiation, common in space, are the main factors influencing astronauts' health in space flight, but their combined effects on immune cells are extremely limited. Therefore, the effect of simulated microgravity on heavy ion radiation-induced apoptosis, and reactive oxygen species (ROS)-sensitive apoptosis signaling were investigated in human B lymphoblast HMy2.CIR cells. Simulated microgravity was achieved using a Rotating Wall Vessel Bioreactor at 37°C for 30 min. Heavy carbon-ion irradiation was carried out at 300 MeV/u, with a linear energy transfer (LET) value of 30 keV/μm and a dose rate of 1Gy/min. Cell survival was evaluated using the Trypan blue exclusion assay. Apoptosis was indicated by Annexin V/propidium iodide staining. ROS production was assessed by cytometry with a fluorescent probe dichlorofluorescein. Malondialdehyde was detected using a kit. Extracellular signal-regulated kinase (ERK), mitogen-activated protein kinase phosphatase-1 (MKP-1) and caspase-3 activation were measured by immunoblotting. Simulated microgravity decreased heavy ion radiation-induced cell survival and increased apoptosis in HMy2.CIR cells. It also amplified heavy ion radiation-elicited intracellular ROS generation, which...Continue Reading

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Citations

Nov 11, 2015·Life Sciences in Space Research·Christine E HellwegGünther Reitz
Apr 2, 2020·International Journal of Molecular Sciences·Chiara De CesariDebora Angeloni
Apr 3, 2020·Journal of Radiation Research·Qiuning ZhangXiaohu Wang
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Dec 15, 2020·Biological Reviews of the Cambridge Philosophical Society·Zhe ShiXin-Min Liu
Dec 16, 2020·International Journal of Molecular Sciences·Binod PrasadMarcus Krüger

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