Simulated microgravity induces a cellular regression of the mature phenotype in human primary osteoblasts

Cell Death Discovery
Magda GioiaMassimo Coletta

Abstract

Decreased mechanical loading on bones, such as prolonged bed rest and microgravity during space flights, leads to the development of an osteoporotic-like phenotype. Although osteoblast hypo-functionality is reported to be involved in the progression of bone pathological conditions, the cellular mechanisms of this process remain largely unknown. The combined application of mass spectrometry "-omics" and histochemical and ultrastructural approaches have been employed to investigate the effects of the gravitational unloading on human bone-cell biology. Here we show, ex vivo, that simulated microgravity (Sμg) on human primary osteoblasts (hpOB) induces an alteration of pro-osteogenic determinants (i.e., cell morphology and deposit of hydroxyapatite crystals), accompanied by a downregulation of adhesive proteins and bone differentiation markers (e.g., integrin beta-1, protein folding Crystallin Alpha B (CRYα-B), runt-related transcription factor 2 (RUNX-2), bone morphogenic protein-2 (BMP-2), and receptor activator of nuclear factor kappa-B ligand (RANK-L)), indicating an impairment of osteogenesis. Further, we observed for the first time that Sμg can trigger a transition toward a mesenchymal-like phenotype, in which a mature osteob...Continue Reading

References

Mar 1, 1983·The American Journal of Physiology·W S JeeD B Kimmel
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Jan 18, 2003·Journal of Cellular Biochemistry·C Ontiveros, Laura R McCabe
May 16, 2003·Nature·Shun-ichi Harada, Gideon A Rodan
Aug 13, 2004·Nature·Dorothy A Schafer
Aug 9, 2005·The Journal of Surgical Research·Kristin BjørnlandGunhild M Maelandsmo
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Feb 18, 2009·Blood·Daniel NowakH Phillip Koeffler
Apr 14, 2009·Trends in Molecular Medicine·Katerina K PapachroniAthanasios G Papavassiliou
Jan 5, 2010·Nature Reviews. Rheumatology·Engin OzciviciClinton T Rubin
Sep 14, 2010·Journal of Cellular Physiology·Lorraine J Gudas, John A Wagner
Jan 22, 2011·Nature Reviews. Molecular Cell Biology·Chris JoplingJuan Carlos Izpisua Belmonte
Nov 1, 2011·Trends in Cell Biology·Katarina Wolf, Peter Friedl
May 18, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shikha GuptaStefan Judex
May 23, 2012·Seminars in Cancer Biology·M Angela Nieto, Amparo Cano
Jun 30, 2012·Tissue Engineering. Part a·Kate FrittonStuart B Goodman
Nov 2, 2012·Stem Cell Research & Therapy·Evelyn K F Yim, Michael P Sheetz
Feb 5, 2013·Journal of Cell Science·Ryan J Petrie, Kenneth M Yamada
Apr 17, 2013·Acta Biomaterialia·Mohammad Nahid AndalibJung Yul Lim
Oct 15, 2013·Expert Review of Proteomics·Angelo D'Alessandro, Lello Zolla
Jan 7, 2014·The International Journal of Biochemistry & Cell Biology·Pierre P ElenisteAngela Bruzzaniti
Jul 8, 2014·Current Opinion in Cell Biology·James E Bear, Jason M Haugh
Jul 24, 2014·International Journal of Cancer. Journal International Du Cancer·Joan FernandoIsabel Fabregat
Nov 18, 2014·Journal of Women's Health·Lori Ploutz-SnyderDebra Bemben
Dec 5, 2014·International Journal of Stem Cells·Masoud MalekiElham Ghadirkhomi
Feb 6, 2015·BioMed Research International·Katrin PaulsenOliver Ullrich
Feb 28, 2015·Biochemical and Biophysical Research Communications·Robert T BradyDavid A Hoey
Jun 6, 2015·Trends in Cell Biology·Irena L IvanovskaDennis E Discher
Sep 14, 2015·Current Opinion in Cell Biology·Anne J Ridley
Jun 29, 2016·Current Opinion in Genetics & Development·Ivonne M SehringGilbert Weidinger
Sep 20, 2016·The International Journal of Biochemistry & Cell Biology·I MorathV Orian-Rousseau

❮ Previous
Next ❯

Citations

Mar 15, 2019·Canadian Journal of Physiology and Pharmacology·Ming YangBaorong He
Feb 11, 2020·Frontiers in Physiology·Warren W Burggren
Apr 30, 2020·International Journal of Molecular Sciences·Xiao LinAirong Qian

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
protein folding
GTPase
GTPases
X-ray
biopsies

Software Mentioned

MassMatrix
MASCOT Daemon application
MASCOT
Noram System Six
FunRich
Fun Rich
MAVEN
Compass DataAnalysis
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Hitomi NakamuraEijiro Jimi
© 2022 Meta ULC. All rights reserved