Simulated microgravity promotes monocyte adhesion to rat aortic endothelium via nuclear factor-κB activation

Clinical and Experimental Pharmacology & Physiology
Huan LiuJin Ma

Abstract

Microgravity-induced vascular remodelling may play an important role in post-spaceflight orthostatic intolerance. In this study, we aimed to investigate the effects of simulated microgravity on monocyte adhesion to aortic endothelium in hindlimb unweighted rats and to elucidate the underlying mechanisms associated with this event. Sprague-Dawley rats were subjected to 4-week hindlimb unweighting to simulate microgravity. The recruitment of monocytes to the abdominal aorta was investigated by en face immunofluorescence staining and monocyte binding assays. The expression of the adhesion molecules E-selectin and vascular cell adhesion molecule-1 as well as the cytokine monocyte chemoattractant protein (MCP)-1 was evaluated by immunohistochemical staining, western blot, and quantitative reverse transcription polymerase chain reaction analyses. Additionally, nuclear factor-κB (NF-κB) activation and the messenger RNA expression levels of E-selectin, vascular cell adhesion molecule-1, and MCP-1 were assessed with the administration of an NF-κB inhibitor, pyrrolidine dithiocarbamate. Results showed that simulated microgravity significantly increased monocyte recruitment to the aortic endothelium, protein expression of E-selectin and M...Continue Reading

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Citations

Mar 31, 2017·Pflügers Archiv : European journal of physiology·Yao-Ping ChengJun-Xiang Bao
Jun 28, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Min JiangRan Zhang
Apr 30, 2020·International Journal of Molecular Sciences·Xiao LinAirong Qian
Apr 2, 2021·NPJ Microgravity·Christopher LudtkaJosephine B Allen

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