Apr 11, 2020

Traumatic Joint Injury Induces Acute Catabolic Bone Turnover Concurrent with Articular Cartilage Damage in a Rat Model of Post-Traumatic Osteoarthritis

BioRxiv : the Preprint Server for Biology
T. MaerzKevin C Baker

Abstract

Objective: Assess acute alterations in bone turnover, microstructure, and histomorphometry following noninvasive anterior cruciate ligament rupture (ACLR). Methods: Twelve female Lewis rats were randomized to receive noninvasive ACLR or Sham loading (n=6/group). In vivo microCT was performed at 3, 7, 10, and 14 days post-injury to quantify compartment-dependent subchondral (SCB) and epiphyseal trabecular bone remodeling. Near-infrared (NIR) molecular imaging was used to measure in vivo bone anabolism (800 CW BoneTag) and catabolism (Cat K 680 FAST). Metaphyseal bone remodeling and articular cartilage morphology was quantified using ex vivo microCT and contrast-enhanced microCT, respectively. Calcein-based dynamic histomorphometry was used to quantify bone formation. OARSI scoring was used to assess joint degeneration, and osteoclast number was quantified on TRAP stained-sections. Results: ACLR induced acute catabolic bone remodeling in subchondral, epiphyseal, and metaphyseal compartments. Thinning of medial femoral condyle (MFC) SCB was observed as early as 7 days post-injury, while lateral femoral condyles (LFC) exhibited SCB gains. Trabecular thinning was observed in MFC epiphyseal bone, with minimal changes to LFC. NIR imag...Continue Reading

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Mentioned in this Paper

Diffusion Weighted Imaging
Anatomical Space Structure
Caveolin 3
Pharmacologic Substance
Chemicals
Simulation
Compound (Substance)
Motivation
Signal Transduction

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