Simulation and experimental determination of the online separation of blood components with the help of microfluidic cascading spirals

Biomicrofluidics
Lisa SprengerUrs O Häfeli

Abstract

Microfluidic spirals were used to successfully separate rare solid components from unpretreated human whole blood samples. The measured separation ratio of the spirals is the factor by which the concentration of the rare component is increased due to the Dean effect present in a flow profile in a curved duct. Different rates of dilution of the blood samples with a phosphate-buffered solution were investigated. The diameters of the spherical particles to separate ranged from 2 μm to 18 μm. It was found that diluting the blood to 20% is optimal leading to a separation ratio up to 1.97. Using two spirals continuously placed in a row led to an increase in separation efficacy in samples consisting of phosphate-buffered solution only from 1.86 to 3.79. Numerical investigations were carried out to display the flow profiles of Newtonian water samples and the shear-thinning blood samples in the cross-section of the experimentally handled channels. A macroscopic difference in velocity between the two rheologically different fluids could not be found. The macroscopic Dean flow is equally present and useful to help particles migrate to certain equilibrium positions in blood as well as lower viscous Newtonian fluids. The investigations high...Continue Reading

References

Oct 1, 1969·Physiological Reviews·E W Errill
Dec 24, 2005·Lab on a Chip·Palaniappan SethuMehmet Toner
Apr 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Arjun P Sudarsan, Victor M Ugaz
Aug 4, 2007·Medical & Biological Engineering & Computing·Anna Marcinkowska-GapińskaLeszek Kubisz
Oct 26, 2007·Lab on a Chip·Michael J FuerstmanGeorge M Whitesides
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dino Di CarloMehmet Toner
Feb 16, 2008·Analytical Chemistry·Dino Di CarloMehmet Toner
Mar 21, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·J BertrandS Battu
Apr 28, 2009·Physical Review Letters·Dino Di CarloMehmet Toner
Oct 1, 2009·Lab on a Chip·Sathyakumar S KuntaegowdanahalliIan Papautsky
Oct 14, 2009·Lab on a Chip·Dino Di Carlo
Dec 22, 2009·The Analyst·Thomas SchneiderMaciej Zborowski
Feb 14, 2013·Scientific Reports·Han Wei HouChwee Teck Lim
Aug 21, 2013·Lab on a Chip·Majid Ebrahimi WarkianiJongyoon Han
Sep 4, 2013·Biotechnology Advances·Hadi EsmaeilsabzaliEdward J Park

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Citations

Jul 6, 2016·Lab on a Chip·Mehdi RafeieMajid Ebrahimi Warkiani

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