Simulation of Cl(-) Secretion in Epithelial Tissues: New Methodology Estimating Activity of Electro-Neutral Cl(-) Transporter

Frontiers in Physiology
Kouhei SasamotoYoshinori Marunaka

Abstract

Transcellular Cl(-) secretion is, in general, mediated by two steps; (1) the entry step of Cl(-) into the cytosolic space from the basolateral space across the basolateral membrane by Cl(-) transporters, such as Na(+)-K(+)-2Cl(-) cotransporter (NKCC1, an isoform of NKCC), and (2) the releasing step of Cl(-) from the cytosolic space into the luminal (air) space across the apical membrane via Cl(-) channels, such as cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel. Transcellular Cl(-) secretion has been characterized by using various experimental techniques. For example, measurements of short-circuit currents in the Ussing chamber and patch clamp techniques provide us information on transepithelial ion movements via transcellular pathway, transepithelial conductance, activity (open probability) of single channel, and whole cell currents. Although many investigators have tried to clarify roles of Cl(-) channels and transporters located at the apical and basolateral membranes in transcellular Cl(-) secretion, it is still unclear how Cl(-) channels/transporters contribute to transcellular Cl(-) secretion and are regulated by various stimuli such as Ca(2+) and cAMP. In the present study, we simulate transcellu...Continue Reading

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Citations

Jun 3, 2017·Annals of the New York Academy of Sciences·Yoshinori Marunaka
Aug 15, 2017·International Journal of Molecular Sciences·Yoshinori Marunaka
Nov 30, 2018·International Journal of Molecular Sciences·Taka-Aki InuiTakashi Nakahari
May 16, 2020·International Journal of Molecular Sciences·Rie Marunaka, Yoshinori Marunaka
Apr 17, 2021·PLoS Neglected Tropical Diseases·Jian-Bang XuYi-Lin Zhang

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