Simulation of cyclic dynein-driven sliding, splitting, and reassociation in an outer doublet pair.

Biophysical Journal
C J Brokaw

Abstract

A regular cycle of dynein-driven sliding, doublet separation, doublet reassociation, and resumption of sliding was previously observed by Aoyama and Kamiya in outer doublet pairs obtained after partial dissociation of Chlamydomonas flagella. In the work presented here, computer programming based on previous simulations of oscillatory bending of microtubules was extended to simulate the cycle of events observed with doublet pairs. These simulations confirm the straightforward explanation of this oscillation by inactivation of dynein when doublets separate and resumption of dynein activity after reassociation. Reassociation is augmented by a dynein-dependent "adhesive force" between the doublets. The simulations used a simple mathematical model to generate velocity-dependent shear force, and an independent elastic model for adhesive force. Realistic results were obtained with a maximum adhesive force that was 36% of the maximum shear force. Separation between a pair of doublets is the result of a buckling instability that also initiates a period of uniform sliding that enlarges the separation. A similar instability may trigger sliding initiation events in flagellar bending cycles.

References

Jan 1, 1986·Cell Motility and the Cytoskeleton·C J Brokaw
Jul 1, 1970·Journal of Theoretical Biology·R Rikmenspoel, M A Sleigh
Jan 1, 1983·Cell Motility·C J Brokaw, D J Luck
Aug 23, 2005·Biophysical Journal·Susumu Aoyama, Ritsu Kamiya
Apr 20, 2007·Cell Motility and the Cytoskeleton·Charles B Lindemann, David R Mitchell
Oct 2, 2008·Cell Motility and the Cytoskeleton·Charles J Brokaw
Jun 19, 2009·Cell Motility and the Cytoskeleton·Dominic W PelleCharles B Lindemann

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Citations

Jul 26, 2013·Journal of the Royal Society, Interface·Nicolas Bruot, Pietro Cicuta
Oct 9, 2014·Biophysical Journal·Charles B Lindemann
Dec 2, 2009·Biophysical Journal·Charles B Lindemann
Aug 26, 2014·Reproductive Biology·Viktoriya Dzyuba, Jacky Cosson
Apr 29, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Charles B Lindemann, Kathleen A Lesich
Jun 5, 2014·Biophysical Journal·Charles B Lindemann
Jan 6, 2022·Journal of the Royal Society, Interface·Laura CollesanoAndrej Vilfan

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