Simulation of the cytoskeletal response of cells on grooved or patterned substrates

Journal of the Royal Society, Interface
A VigliottiVikram S Deshpande

Abstract

We analyse the response of osteoblasts on grooved substrates via a model that accounts for the cooperative feedback between intracellular signalling, focal adhesion development and stress fibre contractility. The grooved substrate is modelled as a pattern of alternating strips on which the cell can adhere and strips on which adhesion is inhibited. The coupled modelling scheme is shown to capture some key experimental observations including (i) the observation that osteoblasts orient themselves randomly on substrates with groove pitches less than about 150 nm but they align themselves with the direction of the grooves on substrates with larger pitches and (ii) actin fibres bridge over the grooves on substrates with groove pitches less than about 150 nm but form a network of fibres aligned with the ridges, with nearly no fibres across the grooves, for substrates with groove pitches greater than about 300 nm. Using the model, we demonstrate that the degree of bridging of the stress fibres across the grooves, and consequently the cell orientation, is governed by the diffusion of signalling proteins activated at the focal adhesion sites on the ridges. For large groove pitches, the signalling proteins are dephosphorylated before they...Continue Reading

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Citations

Sep 5, 2015·Biomechanics and Modeling in Mechanobiology·A VigliottiV S Deshpande
Nov 27, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Sandra LoerakkerFrank P T Baaijens
Jul 16, 2017·Scientific Reports·Alessio GizziAlberto Rainer

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