Simulation of the rat intestinal ecosystem using a two-stage continuous culture system

Journal of General Microbiology
B G Veilleux, I Rowland

Abstract

To investigate the ecological mechanisms governing the community structure of the gut microbial ecosystem, we have attempted to simulate the rat gut ecosystem in vitro using a two-stage continuous culture. Extensive sampling of the rat hindgut has established a set of criteria with which the in vitro system may be compared. This paper discusses one of the criteria, the community composition and structure in vivo and in vitro. The experiments indicated that a gut microbial ecosystem could be satisfactorily mimicked in vitro. This was achieved using a two-stage continuous culture employing differential selection of species between the two stages on the basis of pH differences combined with cell recycling between stages.

Associated Clinical Trials

Citations

Dec 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A K MallettS D Gangolli
Jan 1, 1987·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T M CouttsI R Rowland
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Jan 1, 1994·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·L CuzzolinG Benoni
Jan 1, 1992·Critical Reviews in Food Science and Nutrition·C J Rumney, I R Rowland
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Jun 1, 1986·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A K MallettI R Rowland
Feb 1, 1982·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A WiseI R Rowland
Oct 1, 1992·Journal of Chemotherapy·G BenoniM E Fracasso
Jun 1, 1992·Journal of Chemotherapy·L CuzzolinG Benoni

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