Simultaneous acquisition of 2D and 3D solid-state NMR experiments for sequential assignment of oriented membrane protein samples

Journal of Biomolecular NMR
T GopinathG Veglia

Abstract

We present a new method called DAISY (Dual Acquisition orIented ssNMR spectroScopY) for the simultaneous acquisition of 2D and 3D oriented solid-state NMR experiments for membrane proteins reconstituted in mechanically or magnetically aligned lipid bilayers. DAISY utilizes dual acquisition of sine and cosine dipolar or chemical shift coherences and long living (15)N longitudinal polarization to obtain two multi-dimensional spectra, simultaneously. In these new experiments, the first acquisition gives the polarization inversion spin exchange at the magic angle (PISEMA) or heteronuclear correlation (HETCOR) spectra, the second acquisition gives PISEMA-mixing or HETCOR-mixing spectra, where the mixing element enables inter-residue correlations through (15)N-(15)N homonuclear polarization transfer. The analysis of the two 2D spectra (first and second acquisitions) enables one to distinguish (15)N-(15)N inter-residue correlations for sequential assignment of membrane proteins. DAISY can be implemented in 3D experiments that include the polarization inversion spin exchange at magic angle via I spin coherence (PISEMAI) sequence, as we show for the simultaneous acquisition of 3D PISEMAI-HETCOR and 3D PISEMAI-HETCOR-mixing experiments.

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Citations

Feb 10, 2016·Molecular Membrane Biology·Simon G Patching
Oct 22, 2015·The Journal of Physical Chemistry. B·Evgeniy S SalnikovBurkhard Bechinger
Jun 17, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Rongchun ZhangAyyalusamy Ramamoorthy
Jun 3, 2017·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Evgeniy S SalnikovOlivier Ouari
Jan 25, 2019·Magnetic Resonance in Chemistry : MRC·Pau Nolis, Teodor Parella
Jun 13, 2019·The Journal of Membrane Biology·Christopher AisenbreyBurkhard Bechinger
Mar 4, 2021·Accounts of Chemical Research·Tata GopinathGianluigi Veglia
Feb 4, 2019·Chemistry and Physics of Lipids·Evgeniy S SalnikovBurkhard Bechinger
Sep 5, 2021·Progress in Nuclear Magnetic Resonance Spectroscopy·Ēriks KupčeTim D W Claridge

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