Simultaneous analysis of carbohydrates, polyols and amines in urine samples using chemical ionization gas chromatography with tandem mass spectrometry

Journal of Separation Science
Róbert KubinecNgô Mạnh Thắng

Abstract

A simple method for the simultaneous derivatization of carbohydrates, polyols, amines and amino acids using hexamethyldisilazane and N,O-bis(trimethylsilyl)trifluoroacetamide was developed. This method allows the direct derivatization of urine samples without sample pretreatment before derivatization. The method was successfully used for analysis of the selected metabolites in urine samples of healthy individuals and neonates suffering from galactosemia. The limits of detection by positive chemical ionization gas chromatography with tandem mass spectrometry analysis were in the range of 1.0 mgL-1 for mannitol to 4.7 mg/L for glucose.

References

Sep 24, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·T KuharaI Matsumoto
Aug 3, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·K XuI Matsumoto
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tomiko Kuhara
Jan 31, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Peter SchadewaldtUdo Wendel
Dec 13, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Claire YagerStanton Segal
Dec 17, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jeongae Lee, Bong Chul Chung
Jul 14, 2006·Journal of Inherited Metabolic Disease·Annet M Bosch
May 29, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qi ZhangA Jiye
Jun 10, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Harin KananiMaria I Klapa
Nov 11, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Moacir WajnerCarmen Regla Vargas
Dec 28, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A I Ruiz-MatuteI Martínez-Castro
Mar 11, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Konstantina SpagouMaria I Klapa
Sep 21, 2011·Urologic Oncology·Sheila Ganti, Robert H Weiss
Aug 21, 2012·Journal of Forensic Sciences·Cristiana GambelungheRiccardo Rossi
Jan 15, 2014·Journal of Breath Research·B de Lacy CostelloN M Ratcliffe
Feb 1, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chrysoula ChristouGeorgios Theodoridis
Apr 2, 2014·Prostate Cancer and Prostatic Diseases·A D AsimakopoulosE Finazzi-Agrò
Aug 5, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Peter PodolecRadomír Čabala
Nov 2, 2014·Journal of Separation Science·Václav Matěj BierhanzlPeter Podolec

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