Simultaneous analysis of dehydroacetic acid, benzoic acid, sorbic acid and salicylic acid in cosmetic products by solid-phase extraction and high-performance liquid chromatography

Journal of Pharmaceutical and Biomedical Analysis
E MikamiM Nishida

Abstract

A high-performance liquid chromatographic (HPLC) method for simultaneous determination of dehydroacetic acid (DHA), benzoic acid (BA), sorbic acid (SOA) and salicylic acid (SA) was developed for application to cosmetic products. Isocratic reversed-phase HPLC was employed for quantitative analysis using tetra-n-butylammonium (TBA) hydroxide as an ion-pair reagent. Cosmetic samples were purified by solid-phase extraction using Bond-Elut SI cartridges. Four acidic preservatives were eluted with methanol from cartridges. The HPLC assay was carried out using TSK gel ODS-80TM column (5 microm, 150 x 4.6 mm I.D.). The mobile phase consisted of a mixture of water and methanol (65:35, v/v) containing 2.5 mM TBA hydroxide adjusted with phosphoric acid to pH 7.0. The calibration curves of these preservatives showed good linearity with UV detection (235 nm). The correlation coefficients were better than 0.999 in all cases. The lower limits of detection (defined as a signal-to-noise ratio of about 3) were approximately 2.5 ng for DHA, 4.0 ng for BA, 2.0 ng for SOA and 5.5 ng for SA. The procedure described here is simple, selective and is suitable for quality control of finished cosmetic products.

References

Dec 24, 1998·Journal of Pharmaceutical and Biomedical Analysis·E SottofattoriG Tonello

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Citations

Feb 18, 2006·Analytical and Bioanalytical Chemistry·Behzad Haghighi, Reza Dadashvand
Oct 13, 2006·Journal of Fluorescence·Jing FanMaohong Fan
Jun 1, 2005·Journal of Pharmaceutical and Biomedical Analysis·Cheng-Hui LinYeou-Lih Huang
Mar 8, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Yumei ZhangWeibo Cui
Oct 9, 2013·Journal of Chromatographic Science·Airin AoyamaKeiji Kajimura
Nov 13, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jinghua YuShenguang Ge
Feb 18, 2016·Journal of Pharmaceutical Sciences·Thomas ReschkeChristoph Held
May 9, 2013·International Journal of Cosmetic Science·D N de OliveiraR Catharino
Apr 8, 2016·Journal of Pharmaceutical and Biomedical Analysis·Raffaele LecceAlessia Panusa
Jul 31, 2016·Carbohydrate Polymers·Qiaobin Hu, Yangchao Luo
Nov 14, 2016·Talanta·Cristina Román-HidalgoMiguel Ángel Bello-López
Oct 17, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yong HaoXueguang Shao
Mar 16, 2013·International Journal of Molecular Sciences·Siti Farhana Abdul RaofMhd Radzi Abas
Dec 17, 2009·Luminescence : the Journal of Biological and Chemical Luminescence·J Ballesta-ClaverL F Capitán-Vallvey
Feb 3, 2021·ACS Applied Materials & Interfaces·Zhi-Qiang WangPeng Cheng
Apr 11, 2021·The Science of the Total Environment·Eun Chul PackDal Woong Choi
Dec 18, 2020·ACS Synthetic Biology·Tiffany ChienTal Danino

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