Simultaneous analysis of prostanoids using liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
James KapronKevin P Bateman

Abstract

A liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry (LC-FAIMS-MS/MS) semi-quantitative method was developed for the simultaneous determination of prostaglandin (PG) E2, PGD2, PGF(2alpha), 6-keto-PGF(1alpha), and thromboxane (TX) B2. Diluted samples containing these prostanoids and their tetra-deuterium-substituted internal standards were analyzed by LC followed by either selected reaction monitoring (LC-SRM) or FAIMS and selected reaction monitoring (LC-FRM). FAIMS reduced background noise, separated the isobaric ions PGE2 and PGD2, and separated dynamically interchanging TXB2 anomers. This is the first report of the separation of interconverting anomers by FAIMS. Generally, the LC-FRM chromatograms were more selective than the LC-SRM chromatograms. Its potential was demonstrated by analysis of prostanoids in guinea pig lumbar spinal cord homogenate.

References

Feb 14, 1997·Journal of Chromatography. a·Y Z Hsieh, K L Kuo
Sep 24, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·T ObataK Maekawa
Sep 6, 2002·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M TakabatakeM Mizugaki
Oct 9, 2002·Mini Reviews in Medicinal Chemistry·R Baranowski, K Pacha
Jan 22, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kasem NithipatikomWilliam B Campbell
Sep 30, 2003·Rapid Communications in Mass Spectrometry : RCM·Seon Hwa LeeIan A Blair
Dec 15, 2004·Journal of Chromatography. a·Roger Guevremont
Sep 21, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ronald SchmidtGerd Geisslinger

❮ Previous
Next ❯

Citations

Jun 1, 2013·Analytical and Bioanalytical Chemistry·Gustavo de Souza PessôaMarco Aurélio Zezzi Arruda
Jul 31, 2007·Journal of the American Society for Mass Spectrometry·David A BarnettRandy W Purves
Aug 29, 2007·Journal of the American Society for Mass Spectrometry·Alexandre A Shvartsburg, Richard D Smith
Jul 1, 2008·Journal of the American Society for Mass Spectrometry·Alexandre A Shvartsburg, Richard D Smith
Nov 7, 2008·Analytical Chemistry·Alexandre A Shvartsburg, Richard D Smith
Jul 19, 2008·Annual Review of Analytical Chemistry·Bradley L AckermannAjai K Chaudhary
Oct 1, 2014·Journal of the American Society for Mass Spectrometry·Satendra PrasadRandy W Purves
Aug 12, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Axel MieCurt T Reimann
Apr 12, 2007·Expert Review of Proteomics·Bradley L Ackermann, Michael J Berna
Feb 23, 2008·Analytical Chemistry·Samuel I MerenbloomDavid E Clemmer
Feb 18, 2010·International Journal of Mass Spectrometry·Matthew R Schenauer, Julie A Leary
Apr 1, 2010·Drug Testing and Analysis·Sven GuddatWilhelm Schänzer
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Jan 18, 2008·Journal of Mass Spectrometry : JMS·Abu B KanuHerbert H Hill
Feb 8, 2008·Rapid Communications in Mass Spectrometry : RCM·Panos Hatsis, James T Kapron
Oct 18, 2011·Rapid Communications in Mass Spectrometry : RCM·Whitney B ParsonGary J Van Berkel
Apr 29, 2015·Analytical Chemistry·Hulda S JónasdóttirMartin Giera
Dec 20, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Markus Himmelsbach
Feb 6, 2018·Expert Review of Proteomics·Kelly M FultonSusan M Twine
Sep 20, 2017·Progress in Lipid Research·Jean-Marie GalanoJetty Chung-Yung Lee
Apr 25, 2018·Journal of the American Society for Mass Spectrometry·James P Di GiovanniRobert C Murphy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.