Simultaneous Analysis of Saccharides between Fresh and Processed Radix Rehmanniae by HPLC and UHPLC-LTQ-Orbitrap-MS with Multivariate Statistical Analysis

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Shujuan XueYongxian Cheng

Abstract

Radix Rehmanniae (RR) is a kind of herb which is widely used in the clinical and food processing industry. There are four forms of RR used in traditional Chinese medicine practice, which include fresh RR (FRR), raw RR (RRR), processed RR (PRR), and another processed RR (APRR), in which the APRR was processed by nine cycles of repeated steaming and drying. There are a large number of saccharides in RR. However, the differences in content were shown by different processing methods. In this study, an effective method using high-performance liquid chromatography (HPLC) and high-performance liquid chromatography-mass spectrometry (LC-MS) coupled with multivariate statistical analysis to rapidly distinguish different RR samples and validate the proposed chemical conversion mechanism. The datasets of the content of saccharides were subjected to principal component analysis (PCA) and one-way analysis of variance. The results showed that there different changes occurred in the contents of saccharides corresponding to the different processing methods, in which the contents of monosaccharides-namely arabinose, glucose, mannose, and galactose-had an increasing trend or remained relatively stable. However, the contents of fructose and oligo...Continue Reading

References

Aug 28, 2004·Journal of Chromatography. a·Jorge L Chávez-ServínM Carmen López-Sabater
Nov 7, 2007·Analytica Chimica Acta·Sébastien N RonkartMichel Paquot
Apr 15, 2008·Journal of Ethnopharmacology·Ru-Xue ZhangZheng-Ping Jia
Apr 19, 2012·Journal of Agricultural and Food Chemistry·Roberto ConsonniClelia Cogliati
Jan 8, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jin-hua TaoShu Jiang

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Citations

Jan 24, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Bei-Hua BaoLi Zhang
Nov 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jian JinYuhui Qin

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Methods Mentioned

BETA
nuclear magnetic resonance
NMR
light scattering
PCA

Software Mentioned

GraphPad Prism
Mass Frontier
Xcalibur
SPSS Statistics
SIMCA

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