PMID: 9188827May 9, 1997Paper

Simultaneous analysis of several non-steroidal anti-inflammatory drugs in human urine by high-performance liquid chromatography with normal solid-phase extraction

Journal of Chromatography. B, Biomedical Sciences and Applications
T HiraiI Kishi

Abstract

A practical and reproducible high-performance liquid chromatographic method using normal solid-phase extraction has been developed for the simultaneous analysis of twelve non-steroidal anti-inflammatory drugs (NSAIDs) in human urine. A urine specimen mixed with acetate buffer pH 5.0 was purified by solid-phase extraction on a Sep-Pak Silica cartridge. The analyte was chromatographed by a reversed-phase Inertsil ODS-2 column using a phosphate buffer-acetonitrile at pH 5.0 as the mobile phase, and the effluent from the column was monitored at 230 or 320 nm. Absolute recoveries were greater than 73% for all of the twelve NSAIDs. The present method enabled simple manipulation and isocratic HPLC with UV analysis as well as high sensitivity of 0.005 microg/ml for naproxen, and 0.05 microg/ml for sulindac, piroxicam, loxoprofen, ketoprofen, felbinac, fenbufen, flurbiprofen, diclofenac, ibuprofen and mefenamic acid as the quantitation limit in human urine using indomethacin as an internal standard.

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Citations

Dec 11, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Asadollah BeiraghiAkbar Razmara
Aug 21, 2003·International Journal of Pharmaceutics·Liang FangYasunori Morimoto
Aug 6, 2003·Journal of Pharmaceutical and Biomedical Analysis·Lotfi Monser, Frida Darghouth
May 14, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pedro DoradoAdrián LLerena
Jan 1, 2008·Acta Crystallographica. Section E, Structure Reports Online·Waseeq Ahmad SiddiquiMasood Parvez
Mar 4, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Joanna Lenik
Feb 12, 2011·Journal of Pharmaceutical and Biomedical Analysis·Yohei SakaguchiHitoshi Nohta
Mar 20, 2009·Biomedical Chromatography : BMC·Hye Won LeeHye Suk Lee
Jul 21, 2007·Biomedical Chromatography : BMC·R BhushanAparna Mukherjee
Mar 31, 2015·Journal of Chromatographic Science·Bilal Yilmaz, Ali Fuat Erdem
Dec 7, 2010·Journal of Pharmaceutical and Biomedical Analysis·Fabrice KrierPhilippe Hubert
Apr 12, 2005·Journal of Pharmacological Sciences·Masaya FukudaTakaaki Hasegawa
Apr 1, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Hyung Joo KimWoo Seong Kim
Aug 16, 2016·Journal of Pharmaceutical and Biomedical Analysis·Teresa Martinez-SenaFrancesc A Esteve-Turrillas
Dec 4, 2003·Journal of Pharmaceutical Sciences·Liang FangYasunori Morimoto
Jun 25, 2013·Journal of Chromatographic Science·Bilal YilmazAli Fuat Erdem
May 21, 2005·Journal of Pharmaceutical and Biomedical Analysis·Antonella ArestaCarlo G Zambonin
Sep 15, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Koichi SuenamiSusumu Kanno
Apr 4, 2006·Journal of Pharmaceutical and Biomedical Analysis·Antonella ArestaCarlo G Zambonin
Mar 22, 2000·Antisense & Nucleic Acid Drug Development·O KhatsenkoR S Geary
Feb 20, 2007·Journal of Separation Science·Koichi SuenamiSusumu Kanno
Nov 30, 2005·Electrophoresis·Wang-Chou Sung, Shu-Hui Chen
Jan 22, 2011·Journal of Separation Science·Serkan Erdemir, Mustafa Yilmaz
Jul 23, 2020·Analytical and Bioanalytical Chemistry·Noemí Aranda-MerinoRut Fernández-Torres

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