Simultaneous and highly sensitive detection of multiple breast cancer biomarkers in real samples using a SERS microfluidic chip

Talanta
Zhihua ZhengYiping Cui

Abstract

Accurate and quantitative analysis of breast cancer biomarkers is a particularly requisite in the early diagnosis of breast cancers, especially in real samples. Here, we develop a surface enhanced Raman scattering (SERS) immunoassay biosensor based on a microfluidic chip for the simultaneous detection of multiple breast cancer biomarkers in real samples. In such an immunoassay, immuno-Ag aggregates are labeled with different Raman reporters to detect multiple breast cancer biomarkers in real samples. Moreover, to better enhance the Raman signals, self-assembled silver nanoparticles are introduced on the bottom of the microfluidic channels to obtain the SERS active immune-substrate. In order to avoid the crosstalk of SERS signals, we designed a spatially separated detection biosensor by using multi-channels in a microfluidic chip. In the experiments, CA153, CA125 and CEA are selected as the models of breast cancer biomarkers, which can be simultaneously detected of in human serum with an excellently specificity and a high sensitivity. Furthermore, the proposed SERS microfluidic biosensor can be applied to detect the biomarkers in real samples using a linear regression method for the statistical analysis. Our detection results ar...Continue Reading

Citations

Jul 8, 2020·Analytical and Bioanalytical Chemistry·Na LiYiping Cui
Dec 8, 2019·Sensors·Muqsit Pirzada, Zeynep Altintas
Sep 29, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kantima KaewjuaWeena Siangproh
Dec 11, 2019·Talanta·Ruchita ShandilyaPradyumna Kumar Mishra
Dec 24, 2020·Biosensors·Yung-Tsan ChenJian-Jang Huang
Jan 1, 2021·Talanta·Shiva AshooriFarshid Raissi
Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Neda ShahbaziSeyed Morteza Naghib

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