Simultaneous and rapid quantification of microalga biomolecule content using electrochemical impedance spectroscopy.

Biotechnology Progress
Mahmoud Al AhmadSulaiman Al-Zuhair

Abstract

Lipids, proteins, and carbohydrates are the major constituents found in microalga cells, in varying proportions, and these biomolecules find applications in different industries. During microalga cultivation, to efficiently manipulate, control, and optimize the productivity of a specific compound for a specific application, real-time monitoring of these three cell components is essential. In this study, a method using measurement of electrical capacitance was developed to simultaneously determine the lipid, protein, and carbohydrate content of microalga cells without the requirement for any pre-processing steps. The marine microalga Nannochloropsis oculata was cultivated under nitrogen starvation conditions to induce lipid accumulation over a period of 22 days. The correlation between the electrical capacitance of the microalga culture and the intracellular biomolecule content (determined by standard techniques) was investigated, enabling subsequent deduction of microalga intracellular content from electrical capacitance of the culture. The accuracy and precision of the technique were proven by validating an independent sample. The main advantage of the proposed technique is its capability of quantifying microalga composition w...Continue Reading

References

Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Mar 14, 2007·Biotechnology Advances·Yusuf Chisti
Apr 25, 2008·Current Protocols in Immunology·Arya Biragyn, Larry W Kwak
Apr 23, 2013·Analytica Chimica Acta·Yimin Chen, Seetharaman Vaidyanathan
May 30, 2014·Scientific Reports·Mahmoud Al AhmadAli Hilal-Alnaqbi
Oct 31, 2014·Scientific Reports·Mahmoud Al AhmadTahir A Rizvi

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