Simultaneous and sensitive determination of xanthotoxin, psoralen, isoimpinellin and bergapten in rat plasma by liquid chromatography-electrospray ionization mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Wei YangLantong Zhang

Abstract

A sensitive, specific and rapid liquid chromatography-mass spectrometry (LC-MS) method has been developed and validated for the simultaneous determination of xanthotoxin (8-methoxypsoralen), psoralen, isoimpinellin (5,8-dimethoxypsoralen) and bergapten (5-methoxypsoralen) in rat plasma using pimpinellin as an internal standard (IS). The plasma samples were pretreated by protein precipitation with methanol and chromatographic separation was performed on a C(18) column with a mobile phase composed of 1 mmol ammonium acetate and methanol (30:70, v/v). The detection was accomplished by multiple-reaction monitoring (MRM) scanning via electrospray ionization (ESI) source operating in the positive ionization mode. The optimized mass transition ion-pairs (m/z) for quantitation were 217.1/202.1 for xanthotoxin, 187.1/131.1 for psoralen, 247.1/217.0 for isoimpinellin, 217.1/202.1 for bergapten, and 247.1/231.1 for IS. The total run time was 6 min between injections. The calibration curves were linear over the investigated concentration range with all correlation coefficients higher than 0.998. The lower limits of quantitation (LLOQ) of these analytes were less than 1.21 ng/ml. The intra- and inter-day RSD were no more than 9.7% and the r...Continue Reading

References

Dec 4, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lutz BräutigamGerd Geisslinger
Feb 16, 2007·Rapid Communications in Mass Spectrometry : RCM·Yang ZhaoChuan Li
Mar 8, 2008·Fitoterapia·Guo Qiang LiHua Shi Guan

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Citations

May 7, 2013·Journal of Pharmaceutical and Biomedical Analysis·Fangyuan GaoGuorong Fan
Mar 13, 2012·Journal of the Science of Food and Agriculture·Věra SchulzováJana Hajšlová
Mar 27, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yaping Wang, Linfang Huang
Mar 15, 2019·Natural Product Research·Shouwen ZhangJunwei He
Aug 5, 2021·Phytotherapy Research : PTR·Youdan LiangXiaofang Li

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