Simultaneous assay of triazolam and its main hydroxy metabolite in plasma and urine by capillary gas chromatography

Journal of Chromatography
P CoassoloJ P Cano


A gas--liquid chromatographic method for the simultaneous determination of triazolam and its major hydroxy metabolite (1-hydroxymethyltriazolam) in human plasma and urine is described. After addition of two internal standards to the biological fluid, extraction at pH 9, acid washing, back-extraction, and derivatization, the analysis was performed on a wall-coated superior capacity open-tubular (WSCOT) CP-Sil 5 capillary column with electron-capture detection. The detection limit was 0.1-0.2 ng/ml; reproducibility was about 6-7% for plasma concentrations below 1 ng/ml. No interference from other possible minor hydroxy metabolites of triazolam was found. Gas chromatography coupled with mass spectrometry validated the chromatographic results. The method was successfully applied to plasma specimens collected from healthy human volunteers following a single intravenous administration of 1 mg of triazolam or 1-hydroxymethyltriazolam.


Mar 1, 1991·International Journal of Legal Medicine·K KudoY Hida
Jan 1, 1984·Journal of Pharmaceutical and Biomedical Analysis·A Hulshoff, H Lingeman
Aug 1, 1997·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·C W BertholdS E Corey
Oct 1, 1995·Journal of Clinical Psychopharmacology·P D KrobothD E Salazar
Aug 1, 1995·Journal of Clinical Psychopharmacology·P D KrobothR B Smith
Oct 1, 1995·Journal of Clinical Psychopharmacology·R H BarbhaiyaD S Greene
Oct 12, 1990·Journal of Chromatography·A Sioufi, J P Dubois
Mar 20, 1987·Journal of Chromatography·P HeizmannB Ludwig

Related Concepts

Chromatography, Gas-Liquid
Chromatography, Gas-Liquid-Mass Spectrometry
Study, Methodological
Anti-Anxiety Effect

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.