Simultaneous assessment of DNA ploidy and biomarker expression in paraffin-embedded tissue sections

Histopathology
Stijn J H M FleskensJeroen A W M van der Laak

Abstract

Simultaneous assessment of DNA ploidy and biomarker expression in paraffin-embedded tissue sections Aims: Aneuploidy is a potential biomarker for predicting progression of premalignancies. Ploidy assessment is mostly performed on nuclei isolated from tissue sections. Ploidy assessment in situ in tissue sections may be a large improvement, enabling selective sampling of nuclei, thus allowing the correlation between ploidy and histology. Existing ploidy analysis methods in sections suffer from limited sensitivity. The aim was to reliably assess ploidy in sections, combined with simultaneous assessment of other markers at the individual cell level. Ploidy was measured in 22 paraffin-embedded oral premalignancies. The DNA stoichiometric Feulgen procedure was used on isolated nuclei, as well as fluoresence in situ hybridization analysis. In tissue sections, Feulgen was combined with immunohistochemistry for Ki67 proliferation marker, enabling distinction between cycling euploid and aneuploid cells. Aneuploidy was reliably detected in tissue sections (sensitivity 100%, specificity 92%). One section in which aneuploidy was detected was misclassified in isolated nuclei analysis. Sections were also successfully analysed using our model ...Continue Reading

References

Nov 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D W HedleyE A Musgrove
Jun 1, 1993·American Journal of Clinical Pathology·Z SapiE J Wilkinson
Jan 15, 1996·Cancer·J K RobinsonC Zoladz
Apr 1, 1997·Journal of Surgical Oncology·L G DoddL J Layfield
Apr 14, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J A VeltmanA H Hopman
Mar 21, 2002·Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology·G HaroskeA Böcking
May 24, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Jesper Reibel
Aug 15, 2003·Gastrointestinal Endoscopy Clinics of North America·Brian J ReidPeter S Rabinovitch
May 1, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Megan M GarrityUNKNOWN North Central Cancer Treatment Group
Nov 19, 2004·Nature·Harith Rajagopalan, Christoph Lengauer
Nov 22, 2005·Journal of Surgical Oncology·Linetta B KoppertWinand N M Dinjens
Apr 29, 2006·Journal of Clinical Pathology·B Perez-OrdoñezR C K Jordan
Aug 4, 2006·Cancer Research·Keith D HunterPaul R Harrison
Aug 26, 2006·Oral Oncology·Kamal-Eldin Ahmed Abou-Elhamd, Tito Naeem Habib
Jan 16, 2007·Experimental Dermatology·Tim SmitsMarie-Jeanne P Gerritsen
Jan 26, 2007·The Journal of Molecular Diagnostics : JMD·Lawrence D True, Xiaohu Gao
Feb 21, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Chenggong YuRaj K Goyal
Mar 13, 2008·Cancer Biomarkers : Section a of Disease Markers·Marjon KerkhofPeter D Siersema
Apr 19, 2008·The Journal of Pathology·V E BergshoeffE-J M Speel
Jun 1, 1986·IEEE Transactions on Pattern Analysis and Machine Intelligence·J Canny

❮ Previous
Next ❯

Citations

Jan 21, 2011·Current Oncology Reports·Paul Nankivell, Hisham Mehanna
Jan 18, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Thijs RoelofsenLeon F A G Massuger
Aug 4, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yinhai WangPeter W Hamilton
Sep 19, 2012·International Journal of Cancer. Journal International Du Cancer·Jeroen J MoorenErnst-Jan M Speel
Oct 1, 2013·Oral Oncology·T J H SiebersP J Slootweg
Aug 2, 2011·Oral Oncology·Sabrina Daniela da SilvaLuiz P Kowalski
Jul 22, 2017·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Celina Faig LimaJanete Dias Almeida
Aug 14, 2019·Scientific Reports·Altuna HalilovicPeter Bult

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.