PMID: 11180071Feb 17, 2001Paper

Simultaneous catalysis and product separation by cross-linked enzyme crystals

Biotechnology and Bioengineering
M LeisolaO Pastinen

Abstract

Crystalline cross-linked xylose isomerase (CLXI, EC 5.3.1.5) and xylanase (CLX, EC 3.2.1.8) were studied in a packed-bed reactor for simultaneous catalytic reaction and separation of substrates from reaction products. Streptomyces rubiginosus xylose isomerase catalyzed a slow isomerization of L-arabinose to L-ribulose and an epimerization to L-ribose. In equilibrium the reaction mixture contained 52.5% arabinose, 22.5% ribulose, and 25% ribose. In a packed-bed column filled with CLXI, a simultaneous reaction and separation resulted in fractions where arabinose concentration varied between 100-0%, ribulose between 0-55%, and ribose between 0-100%. Trichoderma reesei xylanase II hydrolyzed and transferred xylotetraose mainly to xylotriose and xylobiose. In a packed-bed column filled with CLX, xylotetraose rapidly reacted to xylobiose and xylose by a mechanism that is not yet fully understood.

References

Jul 9, 1999·Biotechnology and Bioengineering·K VisuriM Leisola
Apr 20, 2000·Enzyme and Microbial Technology·O PastinenM Leisola
Nov 1, 1987·Biotechnology and Bioengineering·K Visuri, A M Klibanov

Citations

Aug 30, 2011·Applied Microbiology and Biotechnology·Chao HuCuiying Hu
Jul 27, 2005·Bioprocess and Biosystems Engineering·F TmejP Hasal
May 19, 2009·Journal of Chromatography. a·Zhongqiao Hu, Jianwen Jiang
Nov 30, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Luke F Hartje, Christopher D Snow
Jan 18, 2008·The Journal of Physical Chemistry. B·Kourosh Malek, Marc-Olivier Coppens

Related Concepts

xylobiose
Glucose isomerase
Ribulose, (erythro-L)-isomer
Arabinose
Bacterial Proteins
Catalysis
Polymorphism, Crystallization
Disaccharides
Yeast Proteins
Oligosaccharides

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