PMID: 11180071Feb 17, 2001Paper

Simultaneous catalysis and product separation by cross-linked enzyme crystals

Biotechnology and Bioengineering
M LeisolaO Pastinen


Crystalline cross-linked xylose isomerase (CLXI, EC and xylanase (CLX, EC were studied in a packed-bed reactor for simultaneous catalytic reaction and separation of substrates from reaction products. Streptomyces rubiginosus xylose isomerase catalyzed a slow isomerization of L-arabinose to L-ribulose and an epimerization to L-ribose. In equilibrium the reaction mixture contained 52.5% arabinose, 22.5% ribulose, and 25% ribose. In a packed-bed column filled with CLXI, a simultaneous reaction and separation resulted in fractions where arabinose concentration varied between 100-0%, ribulose between 0-55%, and ribose between 0-100%. Trichoderma reesei xylanase II hydrolyzed and transferred xylotetraose mainly to xylotriose and xylobiose. In a packed-bed column filled with CLX, xylotetraose rapidly reacted to xylobiose and xylose by a mechanism that is not yet fully understood.


Jul 9, 1999·Biotechnology and Bioengineering·K VisuriM Leisola
Apr 20, 2000·Enzyme and Microbial Technology·O PastinenM Leisola
Nov 1, 1987·Biotechnology and Bioengineering·K Visuri, A M Klibanov


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