Simultaneous chemometric determination of pyridoxine hydrochloride and isoniazid in tablets by multivariate regression methods

Drug Testing and Analysis
E DinçD Baleanu

Abstract

The sole use of pyridoxine hydrochloride during treatment of tuberculosis gives rise to pyridoxine deficiency. Therefore, a combination of pyridoxine hydrochloride and isoniazid is used in pharmaceutical dosage form in tuberculosis treatment to reduce this side effect. In this study, two chemometric methods, partial least squares (PLS) and principal component regression (PCR), were applied to the simultaneous determination of pyridoxine (PYR) and isoniazid (ISO) in their tablets. A concentration training set comprising binary mixtures of PYR and ISO consisting of 20 different combinations were randomly prepared in 0.1 M HCl. Both multivariate calibration models were constructed using the relationships between the concentration data set (concentration data matrix) and absorbance data matrix in the spectral region 200-330 nm. The accuracy and the precision of the proposed chemometric methods were validated by analyzing synthetic mixtures containing the investigated drugs. The recovery results obtained by applying PCR and PLS calibrations to the artificial mixtures were found between 100.0 and 100.7%. Satisfactory results obtained by applying the PLS and PCR methods to both artificial and commercial samples were obtained. The resu...Continue Reading

References

Jul 19, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Erdal Dinç, Dumitru Baleanu
Mar 17, 2007·Journal of Drug Targeting·Susan D Tiukinhoy-LaingMelvin E Klegerman
Apr 1, 2010·Drug Testing and Analysis·Mohammad A El-Sayed, Mohammad Abdul-Azim Mohammad

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Citations

Apr 20, 2011·Analytical Chemistry·Jerome WorkmanMel Koch

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