Simultaneous compensation for spatial and temporal dispersion of acousto-optical deflectors for two-dimensional scanning with a single prism

Optics Letters
Shaoqun ZengQingming Luo

Abstract

The dispersive nature of the acousto-optical deflector (AOD) presents a challenge to applications of two sequential orthogonal AODs (a two-dimensional AOD) as XY scanners in multiphoton microscopy. Introducing a prism before the two-dimensional (2D) AOD allows both temporal and spatial dispersion to be compensated for simultaneously. A 90 fs laser pulse was broadened to 572 fs without compensation, and 143 fs with compensation. The ratio of long axis to short axis of the exiting laser beam spot was 3.50 without compensation and 1.14 with compensation. The insertion loss was 37%. Two-photon fluorescence microscopy used the compensated 2D AOD scanner to image a fluorescent microsphere, which improves signal intensity -15-fold compared with the uncompensated scanner.

References

Sep 27, 2002·Biophysical Journal·James D LechleiterIlse Sieneart
Mar 5, 2004·Journal of Neurophysiology·Robert D RoordaGero Miesenböck
May 1, 1984·Optics Letters·R L ForkJ P Gordon
Feb 1, 1988·Optics Letters·M NakazawaH Kubota

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Citations

Sep 4, 2009·The Review of Scientific Instruments·Ramón CarrilesJeffrey A Squier
May 9, 2012·The Review of Scientific Instruments·Runhua JiangShaoqun Zeng
May 5, 2007·Journal of Biomedical Optics·Shaoqun ZengQingming Luo
May 10, 2008·Journal of Biomedical Optics·Zongzhen MaoZhenzhen Han
Jan 12, 2010·Journal of Biomedical Optics·Bradley E LosavioPeter Saggau
May 13, 2010·Journal of Biomedical Optics·Dan ZhuQingming Luo
Apr 25, 2008·Nature Neuroscience·Gaddum Duemani ReddyPeter Saggau
Jun 27, 2009·Annual Review of Neuroscience·Brian A WiltMark J Schnitzer
Sep 15, 2012·Nature Protocols·Xiaowei ChenArthur Konnerth
Mar 18, 2008·Journal of Bioscience and Bioengineering·Xiuli LiuWei Zhou
Sep 12, 2006·Current Opinion in Neurobiology·Peter Saggau
Jun 6, 2014·Scientific Reports·Jae Won ChaPeter T C So
Feb 16, 2007·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Carlos J Zapata-Rodríguez
Mar 7, 2007·Optics Letters·Carlos J Zapata-RodríguezJuan A Monsoriu
Nov 26, 2009·Journal of Biophotonics·Xiang WenDan Zhu
Nov 6, 2009·Physics in Medicine and Biology·Xiang WenDan Zhu
Jul 21, 2010·Journal of Neural Engineering·Bradley E LosavioPeter Saggau
Mar 16, 2007·Applied Optics·Balázs RózsaRóbert Szipocs
Mar 18, 2015·Biomedical Optics Express·Xiuli LiuShaoqun Zeng
Nov 7, 2014·Journal of Biomedical Optics·Wei YanHanben Niu
Jul 20, 2010·Optics Express·Xiaobao LiangLing Fu
Sep 24, 2015·Optics Letters·Zhenqiao ZhouShaoqun Zeng
Feb 19, 2019·Biomedical Optics Express·Ee Zhuan ChongMartin J Booth
May 23, 2008·Analytical Chemistry·Mark LowryIsiah M Warner
Jan 4, 2013·Cold Spring Harbor Protocols·Gaddum Duemani Reddy, Peter Saggau
Jun 4, 2014·Cold Spring Harbor Protocols·Benjamin F Grewe, Fritjof Helmchen

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