Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria

Canadian Journal of Physiology and Pharmacology
Melissa NeoDavid A Saint

Abstract

We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 × 10 silver chloride electrodes, 0.1 mm diameter and 0.1 mm pitch). A glass microelectrode (100 MΩ) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease.

References

Mar 1, 1992·Alcohol·O R CarrylR G Carpentier
Jun 1, 1974·The American Journal of Physiology·F V CavotoJ Roberts
Mar 27, 2004·Physiological Reviews·André G Kléber, Yoram Rudy
Apr 29, 2004·Nature Neuroscience·György Buzsáki
Mar 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Kevin J Sampson, Craig S Henriquez
Aug 24, 2005·Circulation·James N WeissAlain Karma
Oct 18, 2005·Journal of Electrocardiology·Bonnie B PunskeSheldon E Litwin
Oct 6, 2007·Journal of Cardiovascular Electrophysiology·Chiung-Yin ChungEmilia Entcheva
Dec 6, 2007·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Lance D Wilson, David S Rosenbaum
Jul 28, 2010·Clinical and Experimental Pharmacology & Physiology·Dennis H LauPrashanthan Sanders
Mar 20, 2012·American Journal of Physiology. Heart and Circulatory Physiology·David BenoistEd White

❮ Previous
Next ❯

Citations

Feb 26, 2019·International Heart Journal·Boyuan FanQiangsun Zheng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

Cardiac Conduction System

The cardiac conduction system is a specialized tract of myocardial cells responsible for maintaining normal cardiac rhythm. Discover the latest research on the cardiac conduction system here.