Simultaneous cross-linking of CD6 and CD28 induces cell proliferation in resting T cells

Immunology
L M OsorioS C Chow

Abstract

In the present study, we showed that simultaneous ligation of the monoclonal antibodies (mAb) against CD6 and CD28 induces T-cell proliferation in purified resting T lymphocytes in the absence of T-cell receptor (TCR) occupancy. No cell proliferation was observed when the mAb were cross-linked alone or used simultaneously in the soluble form. T-cell proliferation mediated through CD6/CD28 is accompanied by the up-regulation of interleukin-2 (IL-2) mRNA and expression of IL-2 receptors on the cell surface. In the presence of IL-2-neutralizing mAb the proliferative response of the T cell induced through CD6/CD28 was inhibited dose dependently. Cross-linking mAb to CD6 and CD28 alone or together did not down-regulate the CD3/TCR complex. T-cell proliferation mediated through CD6/CD28 was only partially blocked by the immunosuppressive drug, cyclosporin A (CsA), whereas anti-CD28-induced T-cell proliferation in the presence of the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), was unaffected. In sharp contrast T-cell proliferation mediated by anti-CD6 in the presence of TPA was efficiently blocked by CsA. In addition, two protein kinase C (PKC) inhibitors, GF 109203X and H-7 dose-dependently inhibited T-cell proliferati...Continue Reading

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Citations

Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaoyan ShiXiaojing Ma
Jul 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna IbáñezFrancisco Lozano
Apr 10, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Mafalda Pinto, Alexandre M Carmo
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A SkapenkoH Schulze-Koops
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Idoia GimferrerFrancisco Lozano
Dec 11, 2002·The Journal of Biological Chemistry·Idoia GimferrerFrancisco Lozano
Oct 26, 2018·Frontiers in Immunology·Marjolein B M MeddensAlessandra Cambi
Aug 13, 2004·Genes and Immunity·D M EvansN G Martin
Jan 1, 2010·Arthritis·Ruby Alonso-RamirezYves Renaudineau
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Idoia GimferrerFrancisco Lozano

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