Simultaneous dendritic voltage and calcium imaging and somatic recording from Purkinje neurons in awake mice

Nature Communications
Christopher J Roome, Bernd Kuhn

Abstract

Spatiotemporal maps of dendritic signalling and their relationship with somatic output is fundamental to neuronal information processing, yet remain unexplored in awake animals. Here, we combine simultaneous sub-millisecond voltage and calcium two-photon imaging from distal spiny dendrites, with somatic electrical recording from spontaneously active cerebellar Purkinje neurons (PN) in awake mice. We detect discrete 1-2 ms suprathreshold voltage spikelets in the distal spiny dendrites during dendritic complex spikes. Spikelets and their calcium correlates are highly heterogeneous in number, timing and spatial distribution within and between complex spikes. Back-propagating simple spikes are highly attenuated. Highly variable 5-10 ms voltage hotspots are localized to fine dendritic processes and are reduced in size and frequency by lidocaine and CNQX. Hotspots correlated with somatic output but also, at high frequency, trigger purely dendritic calcium spikes. Summarizing, spatiotemporal signalling in PNs is far more complex, dynamic, and fine scaled than anticipated, even in resting animals.

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Citations

Mar 21, 2019·Frontiers in Cellular Neuroscience·Peter QuickeThomas Knöpfel
May 6, 2020·The FEBS Journal·Gen OhtsukiAkitoshi Ozaki
Oct 2, 2019·Frontiers in Systems Neuroscience·Yunliang Zang, Erik De Schutter
Aug 6, 2019·Frontiers in Cellular Neuroscience·Bernd Kuhn, Christopher J Roome
Dec 22, 2020·ELife·Christopher J Roome, Bernd Kuhn
Jan 1, 2021·Frontiers in Cellular Neuroscience·Kevin DorgansMarylka Yoe Uusisaari
May 30, 2020·Neuroscience Research·Tatsuo K Sato
Dec 31, 2020·STAR Protocols·Sigita Augustinaite, Bernd Kuhn
Jan 24, 2021·Journal of Neuroscience Methods·Philipp FlothoDaniel J Strauss
Mar 23, 2021·Cerebral Cortex·Angelique C PaulkSydney S Cash
Mar 23, 2021·Biophysics Reviews·Vincent R DariaHans-A Bachor
Apr 15, 2021·PLoS Computational Biology·Changjia CaiAndrea Giovannucci
May 7, 2021·Science Advances·Miao-Ping ChienAdam E Cohen
Jun 22, 2021·Frontiers in Computational Neuroscience·Yehuda Roth
Jul 20, 2021·Neuroscience·George StuytLucy M Palmer
Aug 5, 2021·Journal of Neurophysiology·Alexander S FanningJennifer L Raymond
Aug 20, 2021·Development, Growth & Differentiation·Kanae HiyoshiSachiko Tsuda
Dec 12, 2021·Nature Communications·Yuki BandoRafael Yuste

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Methods Mentioned

BETA
imaging techniques

Software Mentioned

MathWorks
6plus
ANNINE6
ScanImage
ImageJ
ANNINE
Spike2
Matlab

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