Simultaneous Denoising, Deconvolution, and Demixing of Calcium Imaging Data

Neuron
Eftychios A PnevmatikakisLiam Paninski

Abstract

We present a modular approach for analyzing calcium imaging recordings of large neuronal ensembles. Our goal is to simultaneously identify the locations of the neurons, demix spatially overlapping components, and denoise and deconvolve the spiking activity from the slow dynamics of the calcium indicator. Our approach relies on a constrained nonnegative matrix factorization that expresses the spatiotemporal fluorescence activity as the product of a spatial matrix that encodes the spatial footprint of each neuron in the optical field and a temporal matrix that characterizes the calcium concentration of each neuron over time. This framework is combined with a novel constrained deconvolution approach that extracts estimates of neural activity from fluorescence traces, to create a spatiotemporal processing algorithm that requires minimal parameter tuning. We demonstrate the general applicability of our method by applying it to in vitro and in vivo multi-neuronal imaging data, whole-brain light-sheet imaging data, and dendritic imaging data.

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Citations

Jan 18, 2016·Neuron·Weijian YangDarcy S Peterka
Mar 15, 2016·Neuron·Kanaka RajanDavid W Tank
May 7, 2016·Neuron·Lucas TheisMatthias Bethge
Nov 1, 2016·Nature Methods·Robert PrevedelAlipasha Vaziri
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May 12, 2016·Current Biology : CB·Michael B Orger
May 19, 2017·Neuron·Tsai-Wen ChenKarel Svoboda
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Feb 24, 2018·Scientific Reports·Sander W KeeminkNathalie L Rochefort
Apr 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Somayyeh Soltanian-ZadehSina Farsiu
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Oct 2, 2019·Hippocampus·John L KubieAndré A Fenton
Sep 25, 2019·Nature Neuroscience·Yanjun SunXiangmin Xu

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