Simultaneous detection and genotyping of three genomic groups of Borrelia burgdorferi sensu lato in Dutch Ixodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes.

Journal of Clinical Microbiology
S G RijpkemaJ F Schellekens

Abstract

We developed a rapid and reliable method for the identification Borrelia burgdorferi sensu lato species in ticks. We used the DNA sequence polymorphism of the spacer region between 5S and 23S rRNA genes, which has been shown to be able to discriminate between eight genomic groups of B. burgdorferi sensu lato (D. Postic, M. Assous, P. A. D. Grimont, and G. Baranton, Int. J. Syst. Bacteriol. 44:743-752, 1994). Spacer DNA was amplified by PCR and was then hybridized to five membrane-bound oligonucleotides. The oligonucleotides were specific for B. burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii, and group VS116. A probe which reacted with all genomic groups of B. burgdorferi sensu lato was also used. Ninety-six ticks collected in the field were destructed by bead beating, and the supernatant was used directly in a PCR. B. burgdorferi sensu lato DNA was detected in 6 of 57 adult ticks (11%) and 9 of 39 nymphs (23%). B. garinii was found in three nymphs and four adults, three nymphs carried B. afzelii, and one adult and one nymph carried group VS116. Double infections with B. afzelii and group VS116 were found in two nymphs and one adult. Thus, our method can simultaneously identify three genomic groups of B. burgdorfe...Continue Reading

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Citations

Jun 18, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M CincoF Taggi
Dec 19, 2003·Avian Pathology : Journal of the W.V.P.A·Alicja GryczyńskaMarek Siemiatkowski
Nov 5, 2005·Applied and Environmental Microbiology·Carolin Rauter, Thomas Hartung
Oct 13, 2006·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·B MantelliA Rizzoli
Jun 23, 2007·Emerging Infectious Diseases·Jasmin SkuballaHorst Taraschewski
Dec 25, 2007·Applied and Environmental Microbiology·Veronika Taragel'ováMilan Labuda
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Jun 20, 2012·Emerging Infectious Diseases·Ana M PalomarJosé A Oteo
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Jul 3, 2015·Vector Borne and Zoonotic Diseases·Ilaria PascucciCesare Cammà
Feb 24, 2001·Annals of the New York Academy of Sciences·O SparaganoF Jongejan
May 7, 2003·Applied and Environmental Microbiology·Susanne EttiKlaus Kurtenbach
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Apr 20, 2006·Journal of Medical Entomology·Luigi BertolottiAlessandro Mannelli
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Dec 1, 2002·The Veterinary Quarterly·C M ButlerJ H van der Kolk
Mar 13, 2012·Applied and Environmental Microbiology·R De SousaM S Núncio

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