Simultaneous detection and identification of Candida, Aspergillus, and Cryptococcus species by reverse line blot hybridization

Journal of Clinical Microbiology
E Geoffrey PlayfordTania C Sorrell

Abstract

We report on a reverse line blot (RLB) assay, utilizing fungal species-specific oligonucleotide probes to hybridize with internal transcribed spacer 2 region sequences amplified using a nested panfungal PCR. Reference and clinical strains of 16 Candida species (116 strains), Cryptococcus neoformans (five strains of Cryptococcus neoformans var. neoformans, five strains of Cryptococcus neoformans var. grubii, and six strains of Cryptococcus gatti), and five Aspergillus species (68 strains) were all correctly identified by the RLB assay. Additional fungal species (16 species and 26 strains) not represented on the assay did not exhibit cross-hybridization with the oligonucleotide probes. In simulated clinical specimens, the sensitivity of the assay for Candida spp. and Aspergillus spp. was 10(0.5) cells/ml and 10(2) conidia/ml, respectively. This assay allows sensitive and specific simultaneous detection and identification of a broad range of fungal pathogens.

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Citations

May 18, 2007·Journal of Clinical Microbiology·Shiang Ning LeawTsung Chain Chang
Feb 27, 2009·Journal of Clinical Microbiology·Kizee A EtienneS Arunmozhi Balajee
Jul 27, 2010·Canadian Journal of Microbiology·José Júlio Costa SidrimMarcos Fábio Gadelha Rocha
Mar 30, 2010·Clinics in Dermatology·Roderick J Hay, Rachael Morris Jones
Jul 5, 2012·FEMS Microbiology Ecology·Galina KochkinaDavid Gilichinsky
Feb 15, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A LefortUNKNOWN French Mycosis Study Group
Nov 29, 2011·Indian Journal of Medical Microbiology·P DeshpandeC Rodrigues

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